北京中医药大学学报2016，Vol.39Issue(6)：470-475,6.DOI:10.3969/j.issn.1006-2157.2016.06.007
左归降糖解郁方对糖尿病并发抑郁症大鼠海马区星形胶质细胞谷氨酸转运体的影响
Effect of Zuogui Jiangtang Jieyu Formula on amino acid transporter expression in hippocampal astrocytes of diabetes mellitus rats with depres-sion
摘要
Abstract
Objective To investigate the effects of Zuogui Jiangtang Jieyu Formulation(ZGJTJYF)on the expression of excitatory amino acid transporter 3 (EAAT 3 )and vesicular glutamate transporter 2 (VGLUT2)inhippocampalastrocytesofdiabetesmellitusratswithdepression(DD).Methods DD rats were induced by intragastrical administration of high-fat diet,tail intravenous injection of streptozoto-cin (STZ)with 28 days’chronic stress and were randomly divided into five groups—model group,posi-tive drug group (metformin with fluoxetine),high-,middle-,and low- dose of ZGJTJYF (high-dose, mid-dose,and low-dose)group.Healthy SD rats were assigned into blank control group.After four con-secutive weeks the cognition ability was measured using Morris water maze,and the expression of EAAT3 and VGLUT2 weredetected by using immunohistochemical double labeling method.Results Compared with blank control group,escape latency of model group was significantly longer (P<0.01 ),EAAT 3 expression decreased,VGLUT 2 expression increased remarkably (both P<0.05).Compared with mod-el group,escape latency of positive drug group and in high-dose group decreased significantly (P <0.01),expression of EAAT3 in positive drug group and all ZGJTJYF groups increased(P<0.05),and VGLUT2 expression in positive drug group and high -dose group decreased significantly (P<0.05 ). Conclusion Zuogui Jiangtang Jieyu Formula can improve the disorder of EAAT3 expression and VGLUT2 expression in hippocampal astrocytes of diabetes mellitus rats with depression.关键词
左归降糖解郁方/糖尿病并发抑郁症/星形胶质细胞/兴奋性氨基转运体3/囊泡谷氨酸转运体2/大鼠Key words
Zuogui Jiangtang Jieyu Formula/diabetes mellitus with depression/astrocytes/excitatory amino acid transporter 3 (EAAT 3)/vesicular glutamate transporter 2 (VGLUT 2)/rats分类
医药卫生引用本文复制引用
徐雅岚,赵洪庆,杜青,杨蕙,孟盼,王宇红..左归降糖解郁方对糖尿病并发抑郁症大鼠海马区星形胶质细胞谷氨酸转运体的影响[J].北京中医药大学学报,2016,39(6):470-475,6.基金项目
国家自然科学基金资助项目（No．81573965、No．81403379），国家自然青年科学基金项目（No．81403387），湖南省中药粉体与创新药物省部共建国家重点实验室培育基地开放基金项目 （）
