北京中医药大学学报2016，Vol.39Issue(6)：489-493,5.DOI:10.3969/j.issn.1006-2157.2016.06.010
蒙药复方森登-4特征图谱及其中6个指标性成分的含量测定
Specific chromatogram of Mongolian medicine compound Sendeng-4 and simultaneous determination of its six index components
摘要
Abstract
Objective To establish HPLC specific chromatogram of Sendeng-4,a Mongolian medicine compound,and to simultaneously determine six index components—gallic acid,genipin-1-β-D-gentiobio-side,geniposide,(2R,3R)-dihydromyricetin,rutin,andmyricetin.Methods HPLCspecificchro-matogram was obtained under the following system:Extend-C18 column (250 mm ×4.6 mm,5 μm), mobile phase of acetonitrile (A)and 0.6 % phosphoric acid aqueous solution (B),gradient elution, flow rate at 1 mL/min,the column temperature at 25 ℃,detection wavelength at 254 nm,and the injec-tion volume 10 μL.HPLC system for determination was listed as the following:Extend-C18 column (250 mm ×4.6 mm,5 μm),mobile phase of acetonitrile (A)and 0.6%phosphoric acid aqueous solution (B),gradient elution,flow rate at 0.5 mL/min,the column temperature at 27 ℃,the detection wave-lengthsat254nmand292nm,andtheinjectionvolume10μL.Results Morethan90peakswerede-tected on HPLC specific chromatogram,from which ten were identified by comparing with standard HPLC chromatogram and six were measured.After determination,the content and the peak area of each compo-nent had a good linear relationship,with correlation coefficients 0.999 9,average recovery of 98.29%~103.75%andRSD1.44%~2.97%.Conclusion The method established is accurate,simpleandre-peatable,and can be used to control the quality of Mongolian medicine compound Sendeng-4.关键词
蒙药复方森登-4/特征图谱/没食子酸/京尼平-1-β-D-龙胆二糖苷/栀子苷/(2 R,3 R)-双氢杨梅素/芦丁/杨梅素/高效液相色谱法Key words
Mongolian medicine Sendeng-4/specific chromatogram/gallic acid/genipin-1-β-D-gentiobi-oside/geniposide/(2R,3R)-dihydromyricetin/rutin/myricetin/HPLC分类
医药卫生引用本文复制引用
白埔,萨础拉,董玉..蒙药复方森登-4特征图谱及其中6个指标性成分的含量测定[J].北京中医药大学学报,2016,39(6):489-493,5.基金项目
国家自然科学基金资助项目（No．81160550），内蒙古自治区自然科学基金项目（No．2013JQ03），内蒙古医科大学“英才培育”项目 （）
