川北医学院学报2016，Vol.31Issue(3)：331-335,5.DOI:10.3969/j.issn.1005-3697.2016.03.013
强的松增强 HBV 感染小鼠体内病毒复制表达
Prednisone enhancing viral replication and expression in HBV transfection mice
摘要
Abstract
Objective:To detect the effect of glucorticoid on HBV replication of mice in vivo.Methods:HBV replication com-petent plasmid pAAV /HBV1 .2 was transferred into male C57L /6 mice by hydrodynamics-based in vivo transfection.24 h after transfec-tion,the mice were matched by body weight,age and serum level of Hepatitis B e antigen (HBeAg)and were divided into experiment group and control group,and then treated orally with prednisone or normal saline at interval of twenty four hours for 3 and 10 days.Four to six hours after the last administration,the mice were killed.HBV-DNA replication intermediates in liver were analyzed by Southern Blot Hybridization.The serum HBsAg and HBeAg were detected by chemiluminescent detection system.Results:HBV-DNA replication intermediates were detected in the livers of mice after treating orally with prednisone or normal saline for 3 days,the level of HBV-DNA replication intermediates of the experimental group was 0.127 times higher than that of the control group.However,after treatment for 10 days,compared with the control group of mice,the level of HBV-DNA replication intermediates of the experimental mice increased greatly.Quantitative analysis showed that prednisone enhanced the level of HBV-DNA replication intermediates appropriately 1 .534 fold.Three days after the above treatment,the serum HBsAg of the control mice and the experimental mice were 1 618.28 μg /μL and 1 666.23 μg /μL on average respectively,which showed no significant difference.Similarly,there was no significant difference in the se-rum HBeAg,which showed as follows:14.68 μg /μL,14.95 μg /μL on average respectively.In contrast,10 days after the same treat-ment,the serum HBsAg of the control and the experimental of mice were on average 8 059.40 μg /μL,1 1 885.87 μg /μL,respectively, and the serum HBeAg were 183.15 μg /μL,286.14 μg /μL,respectively.Both of the level of serum HBsAg and HbeAg from experi-mental mice were higher than that of control animals.Conclusion:The replication and expression of hepatitis B virus in the HBV infec-tion mice could be increased by prednisone orally and the increasing effect of prednisone on HBV is time-dependant,which provides fur-ther research about HBV reactivation caused by prednisone and other immunosuppressive drugs,and reminds clinicians,especially non-infected professional doctors to pay full attention to HBV reactivation when glucocorticoid is used in patients with HBV infection.关键词
强的松/乙型肝炎病毒/复制/小鼠模型/高压注射体内转染法Key words
Prednisone/Hepatitis B virus/Replication/Mouse model/Hydrodynamics-based in vivo transfec-tion分类
医药卫生引用本文复制引用
于毅,刘凤君,石瑶,陆明娇,魏小丽..强的松增强 HBV 感染小鼠体内病毒复制表达[J].川北医学院学报,2016,31(3):331-335,5.基金项目
四川省卫生厅项目 （）
