传感技术学报2016，Vol.29Issue(6)：787-796,10.DOI:10.3969/j.issn.1004-1699.2016.06.001
基于氧化石墨烯/聚吡咯-铟锡氧化物微电极的细胞阻抗生物传感器构建及细胞粘附增殖行为检测
Construction of a Cell Impedance Biosensor Based on Graphene Oxide/Polypyrrole-Indium Tin Oxide Micro-Electrode for Detecting Cell Adhesion and Proliferation
摘要
Abstract
This paper describes a cell impedance biosensor based on graphene oxide/polypyrrole-indium tin oxide(GO/PPy-ITO)microelectrode for detecting cell adhesion and proliferation. The ITO microelectrode was firstly fabricated by etching the insulating layer of photosensitive dry film with lithography technology. Then GO/PPy nanocomposite was deposited on the surface of the ITO microelectrode by one-step electropolymerization to form the GO/PPy-ITO micro⁃electrode. Shape measuring laser microscope and scanning electron microscope were used to characterize the surface roughness and topology of the GO/PPy respectively. The electrochemical properties of the GO/PPy-ITO microelectrode were measured by cyclic voltammetry and electrochemical impedance spectroscopy. Human lung cancer cells A 549 adhesion,spreading and proliferation experiments were used to characterize the biocompatibility of the GO/PPy. Used the GO/PPy-ITO microelectrode as sensing electrode,A549 cells adhesion and proliferation were detected by electro⁃chemical impedance spectroscopy. Results showed that the GO/PPy nanocomposite electrodeposited on the surface of the ITO microelectrode had a special structure of smooth surface and abundant micro-pore distribution. Compared with bare ITO microelectrode,GO/PPy-ITO microelectrode had a lower impedance and higher electrochemical activity. Meanwhile,A549 cells cultured on GO/PPy-ITO could attach,spread and proliferate more rapidly than pure PPy. Final⁃ly,as the adhesion and proliferation of A549 cells on the surface of GO/PPy-ITO microelectrode would change the im⁃pedance spectrum characteristics of the electrode system,the information on the cell adhesion and proliferation could be obtained by fitting the impedance spectrum data with the equivalent circuit model. Therefore,as the excellent elec⁃trochemical properties and biocompatibility of the GO/PPy-ITO microelectrode,cell-based impedance biosensor based on this type of microelectrode could be used in the filed such as cellular pathophysiology and drug screening.关键词
生物传感器/电化学阻抗检测/氧化石墨烯/聚吡咯/铟锡氧化物/细胞增殖Key words
biosensor/electrochemical impedance detection/graphene oxide/polypyrrole/Indium tin oxide/cell proliferation分类
信息技术与安全科学引用本文复制引用
李远,张晶,廖娟,王条敏,刘北忠,于超,张玲玲..基于氧化石墨烯/聚吡咯-铟锡氧化物微电极的细胞阻抗生物传感器构建及细胞粘附增殖行为检测[J].传感技术学报,2016,29(6):787-796,10.基金项目
重庆市科委自然科学基金计划资助项目 （）
重庆市永川区创新能力建设平台项目（YCSTC，2014bf5001）；重庆医科大学附属永川医院院级研究项目（YJZD201302,YJZQN201534）资助 （）
