重庆医学2016，Vol.45Issue(16)：2176-2179,4.DOI:10.3969/j.issn.1671-8348.2016.16.006
淫羊藿、黄芪、葛根有效组分复方对阿尔茨海默病转基因模型小鼠大脑皮质DM T1表达的影响
Effects of effective fraction of Epimedium,Astragalus,Radix Puerariae on the expression of DMT1 in the cerebral cortex of transgenic mice model of Alzheimer′s disease
摘要
Abstract
Objective To investigate the effects of Epimedium ,Astragalus ,Radix Puerariae on DMT1 expression in the cere‐bral cortex of APPswe/PS1ΔE9 double transgenic mice model of AD .Methods A total of 60 specific‐pathogen‐free male APPswe/PS1ΔE9 double transgenic mice aged 6 months were equally and randomly assigned to model ,Epimedium ,Astragalus ,Radix puerari‐ae ,compound and DFO groups .An additional 10 6‐month‐old C57BL/6J mice served as negative control group .Using immunohisto‐chemistry and molecular biology methods to investigate the effects of a compound combining the effective components of Epimedi‐um ,Astragalus ,Radix puerariae on DMT1 expression in the cerebral cortex of APPswe/PS1ΔE9 double transgenic mice model of AD . Results Immunohistochemical staining results revealed that DM T 1 positive cell did not show in negative control group .DM T1 ex‐pression was higher in model group compared with the negative control group .DMT1 expression was lower in the compound and deferoxamine groups than in the model group .No significant difference was detected in DM T 1 expression between deferoxamine and compound groups .RT‐PCR ,Western blot and immunohistochemical staining results showed no significant difference .Conclusion These compounds can downregulate DMT1 expression and inhibit iron overload in the cerebral cortex of mice with Alzheimer′s dis‐ease ,reduce iron overload induced impairment of the central nervous system .关键词
阿尔茨海默病/转基因动物模型/小鼠/淫羊藿/黄芪/葛根/二价金属离子转运蛋白1Key words
Alzheimer disease/transgenic animal model/mice/epimedium/Astragalus/Radix Puerariae/divalent metal trans-porter 1分类
医药卫生引用本文复制引用
董贤慧,高维娟,贺小平,崔志超,柴锡庆,赵家晴..淫羊藿、黄芪、葛根有效组分复方对阿尔茨海默病转基因模型小鼠大脑皮质DM T1表达的影响[J].重庆医学,2016,45(16):2176-2179,4.基金项目
国家自然科学基金资助项目（81273983）；河北省食品药品监督管理局资助项目（PT2014053）；河北省教育厅青年基金项目（QN2015205）；河北省中医药管理局资助项目（2015152）；河北省卫生和计划生育委员会资助项目（20160008）；河北省高校重点发展学科建设项目；承德市科技局资助项目（201601A022）。 （）
