重庆医学2016，Vol.45Issue(16)：2182-2185,4.DOI:10.3969/j.issn.1671-8348.2016.16.008
弓形虫CDPK5基因多克隆抗血清的制备及功能鉴定
Preparation of the polyclonal antibodies of CDPK 5 gene from toxoplasma gondii and the identification of its functions
摘要
Abstract
Objective Screening the immune polypeptide sequence of toxoplasma (Tg) CDPK5 gene ,which were synthesized and then immunized the New Zealand white rabbit to prepare antiserum ,and identification its function .Methods Bioinformatics a‐nalysis was used to determine the immune peptide of Tg CDPK5 sequence ,which were artificially synthesized to immune white rab‐bit to prepare antiserum .The titers of antibodies were determined by ELISA and the polyclonal antibodies were verified with CD‐KP5 antigen by Western blot .The sub‐cellular localization of Tg CDPK 5 were obtained by immunofluorescence assay .Results 17 bp peptide sequence from the Tg CDPK5 N‐terminal were chosen as immune polypeptide by bioinformatics analysis .Synthetic pep‐tide were used to immune rabbit to obtain polyclonal antiserum .The result showed that the titer of the obtained ployantibody were 1∶640 000 ;Western blot demonstrated that the antiserum could specifically recognize Tg CDPK 5(75 .4 × 103 );Immunofluores‐cence assay revealed this antibody could specifically recognize the endogenous Tg CDPK 5 of Toxoplasma gondii .Conclusion Ac‐cording to the analysis of Tg CDPK5 sequence information ,this study successful obtained Tg CDPK5 polyclonal antibody .关键词
弓形虫/CDPK5基因/多抗血清/免疫荧光Key words
toxoplasma gondii/CDPK5 gene/antiserum/immunofluorescence assy分类
医药卫生引用本文复制引用
钟亮尹,刘思敏,曾智华,徐晓松,卢汉威,周文超,黄演婷,卢景辉,陈思聪..弓形虫CDPK5基因多克隆抗血清的制备及功能鉴定[J].重庆医学,2016,45(16):2182-2185,4.基金项目
广东省医学科学技术研究基金项目（2015120124650789）。 （）
