重庆医学2016，Vol.45Issue(16)：2226-2228,3.DOI:10.3969/j.issn.1671-8348.2016.16.021
PCR-CTPP在DNA 碱基切除修复基因SNP分型中的应用
The role of polymerase chain reaction with confronting two-pair primers in SNP genotyping of DNA base excision repair genes
彭杨 1程燚 1杨宇馨 1李崇义 1李梦侠 1张诗珩 1王东1
作者信息
- 1. 第三军医大学大坪医院野战外科研究所肿瘤中心，重庆400042
- 折叠
摘要
Abstract
Objective It is important to precisely determinate the single nucleotide polymorphisms (SNPs) in many genes in‐cluding genes related with base excision repair (BER) pathway .This research is conducted to evaluate the role of polymerase chain reaction with confronting two‐pair primers (PCR‐CTPP) in analyzing the SNPs of BER pathway .Methods Four common SNPs of BER pathway (OGG1 Ser326Cys ,XRCC1 Arg399Gln ,APE1 Asp148Glu and‐141T/G in the promoter region) was detected with PCR‐CTPP .10 of the products were sent for genotype sequencing .Compare the results of PCR and sequencing to evaluate the accu‐racy of PCR‐CTPP .Results The genotypes were exactly the same as the sequencing .Conclusion The PCR‐CTPP was a reliable and rapid detective technology for SNPs genotyping .Its broadest application would be great help for gene variant analysis .关键词
多态性 ,单核苷酸/两对引物-聚合酶链式反应/碱基切除修复途径Key words
polymorphism ,single nucleotide/polymerase chain reaction with confronting two-pair primers/base excision repair分类
生物科学引用本文复制引用
彭杨,程燚,杨宇馨,李崇义,李梦侠,张诗珩,王东..PCR-CTPP在DNA 碱基切除修复基因SNP分型中的应用[J].重庆医学,2016,45(16):2226-2228,3.
