重庆医学2016，Vol.45Issue(17)：2305-2307,3.DOI:10.3969/j.issn.1671-8348.2016.17.001
M 3受体激动剂对急性缺血性心肌的保护作用
Protective effects of choline on myocardial ischemic rat heart and its potential mechanisms
摘要
Abstract
Objective To study the protective effects of choline on myocardial ischemia rat heart and its potential mecha -nisms .Methods Ischemia hypoxia environment was simulated with low value of pH (pH 6 .8) and lack of oxygen .Calcium currents were recorded by whole cell patch under the voltage clamp configuration .The alternations in[Ca2 + ]induced by KCl was detected by laser scanning confocal microscope in ventricular myocytes ,then disccuss the effects of choline on calcium and calcium store in cells . Results The normalized peak currents of ICa-L in ventricular myocytes were larger in pH 6 .8 group than those in pH 7 .4 group , which can be attenuated by choline .The(Ca2 + )i induced by KCl in ventricular myocytes were significantly increased in pH 6 .8 Ty-rode solution and its increasing can be suppressed by choline .4-DAMP can inhibit the suppressing effect of choline .Conclusion The possible mechanism of M 3 receptor involved in the protection of ischemic myocardium by inhibiting myocardial cells in ICa-L ,in-hibiting intracellular calcium overload .关键词
心肌缺血/胆碱/L-型钙电流/膜片钳/激光扫描共聚焦Key words
myocardial ischemia/choline/ICa-L/patch clamp technique/laser scanning confocal microscope分类
医药卫生引用本文复制引用
栾海蓉,孙健,王得利,李丽,吴红..M 3受体激动剂对急性缺血性心肌的保护作用[J].重庆医学,2016,45(17):2305-2307,3.基金项目
国家自然科学基金青年基金资助项目（81300163）；黑龙江省教育厅科研课题（12531731）；牡丹江医学院科学技术研究项目（ZS201321）。 （）
