重庆医学2016，Vol.45Issue(17)：2330-2333,4.DOI:10.3969/j.issn.1671-8348.2016.17.008
富氢水对大鼠非酒精性脂肪肝的保护作用
Hydrogen-rich saline protects against nonalcoholic fatty liver in rat
摘要
Abstract
Objective To explore the protective effect of Hydrogen-rich saline against nonalcoholic fatty liver and its poten-tial mechanism in rats .Methods Furty-eight SD rats (weight :150 - 180 g) were randomly divided into control group (C) ,non-al-coholic fatty liver group (M ) ,non-alcoholic fatty liver + Hydrogen-rich saline group (M + H2) ,Hydrogen-rich saline group (H2) ;group C and group H2 were fed with conventional breeding ,group M and group(M + H2) were fed with high fat diet + Terramycin (10 mg/100 g .B .W ,time/5 d ,intraperitoneal injection) to establish the model of nonalcoholic fatty liver disease in rats ;four rats were randomly selected from each group after consecutive exposure eight weeks ,the pathology characteristics of liver tissue was tested by HE staining ,and observed that fat cavitation symbolized the model established successfully .The content of alanine amin-otransferase (ALT) ,malondialdehyde (MDA ) and superoxide dismutase (SOD) activity in each rat′s peripheral blood was tested by biochemical methods .From the ninth week ,each group was starting to be fed with conventional breeding ,group (C) and group (M ) were treated with normal saline (4 mL /per/d) ,group (M + H2) and group (H2) were treated with Hydrogen-rich saline (4 mL /per/d) ;by consecutive intraperitoneal injection 24 days later ,observed the change of the pathological features of liver tissue and de-tected the contents of ALT ,MDA ,and SOD activity of peripheral blood in rats .Results After eight weeks ,the result of HE stai-ning showed that there were a lot of fat vacuoles in the pathologic section of liver tissue from group M and group (M + H2) ;in con-trast ,there were no fat vacuoles were observed in liver tissue from group C and group H 2 .Blood tests results were showed that compared with group C ,the content of ALT and MDA significantly increased in group M and group (M + H2) (P< 0 .05) ,and the SOD activity was significantly lower in group M and group (M + H2) (P< 0 .05) .After Hydrogen-rich saline or normal saline in-jection for 24 days ,the results of HE staining showed that only few fat cavitations of rat liver tissue in group (M + H2) was ob-served without obvious damage of the majority liver tissue in this group ;whereas in group M ,a lot of lipid deposition account for more than half of this area in these liver tissues ,and the liver cells were damaged distinctly .Blood tests were showed that compared with group M rats ,the content of ALT ,MDA decreased in group (M + H2) (P< 0 .05) ,and the activity of SOD increased in group (M + H2) (P< 0 .05) ;however ,by comparison ,the content of ALT ,MDA fostered more in group (M + H2) (P< 0 .05) ,and the activity of SOD decreased more percentage in group (M + H2) than those in group C(P< 0 .05) ,respectively .Conclusion Those results suggest that the model of nonalcoholic fatty liver disease was successfully established in rats ,Hydrogen-rich saline treatment has certain protective effect on nonalcoholic fatty liver ,and its underlying mechanism related to the antioxidant effective of rich hy -drogen water probably .关键词
富氢水/非酒精性脂肪肝/脂肪空泡/氧化应激Key words
hydrogen water/nonalcoholic fatty liver disease/fat cavitations/oxidative stress分类
医药卫生引用本文复制引用
张景云,王跃会,李佳炜,毛捷,秦启忠..富氢水对大鼠非酒精性脂肪肝的保护作用[J].重庆医学,2016,45(17):2330-2333,4.基金项目
重庆医科大学创新实验项目（201442）。 （）
