重庆医学2016，Vol.45Issue(17)：2343-2345,3.DOI:10.3969/j.issn.1671-8348.2016.17.012
贵阳地区2013年手足口病非 EV71、非 CVA16型肠道病毒的检测分析
Etiology study of hand,foot and mouth disease related non-EV71,non-CoxA16 enteroviruses in Guiyang area during 2013
摘要
Abstract
Objective To explore the prevalence of non-EV71 ,non-CoxA16 enteroviruses strains of hand ,foot and mouth disease(HFMD) in Guiyang ,and analyze the genetic evolution of these non-EV71 ,non-CoxA16 enteroviruses strain ,and to provide a basis for preventing and controlling (HFMD) in Guiyang .Methods A total of 107 HFMD specimens tested positive for entero-viruses and negative for EV71 and CoxA16 were collected from April to June of 2013 .The virus RNA was extracted ,and the VP4 sequence was amplified by nested RT-PCR .The PCR positive products were sequenced and the enteroviruses type was identified by sequence BLAST .The sequence and phylogenetic analysis of the predominant virus type was performed .Results 100 out of 107 (93% )specimens were tested positive by nested RT-PCR ,among which 100 PCR products were successfully sequenced .And the types of the 100 enterovirus strains were identifled by sequence BLAST :46 CoxA6 ,30 CoxA10 ,10 CoxA5 ,6 CoxA2 ,2 CoxA3 and 2 CoxA6 ,2 CoxB2 and 2 ECHO16 .CoxA6 accounted for 42 .99% in typed non-EV71 ,non-CoxA16 enterovimses ,and was the pre-dominant type .Conclusion Hand ,foot and mouth disease in Guiyang was conceded with diverse enteroviruses ,and CoxA6 was the predominant type among non-EV71 ,non-CoxA16 enteroviruses .关键词
手足口病/肠道病毒/基因型Key words
hand ,foot and mouth disease/enterovirus/genotype分类
医药卫生引用本文复制引用
杨兴林,梁跃东,洪章萍,熊金凤,王云芬,黄海..贵阳地区2013年手足口病非 EV71、非 CVA16型肠道病毒的检测分析[J].重庆医学,2016,45(17):2343-2345,3.基金项目
贵阳市科技局项目（筑科合［2013103］9号）；贵阳市高层次创新型青年卫生人才项目（筑卫合［2013］创2号）。 （）
