重庆医学2016，Vol.45Issue(18)：2475-2477,3.DOI:10.3969/j.issn.1671-8348.2016.18.008
慢性缺血性痴呆大鼠大脑皮层及海马中 HO-1、VEGF表达的变化
Changes of VEGF and HO-1 expression in cerebral cortex and hippocampus of chronic ischemic vascular dementia rat
沈瑞乐 1常文广 2吴艳芝 3滕军放4
作者信息
- 1. 河南科技大学第一附属医院神经内科，河南洛阳471003
- 2. 新乡市中心医院神经内科，河南新乡453000
- 3. 郑州市中心医院神经内科，郑州450052
- 4. 郑州大学第一附属医院神经内科，郑州450052
- 折叠
摘要
Abstract
Objective To investigate the changes of learning and memory function ,vascular endothelial growth factor (VEGF) and heme oxygenase‐1(HO‐1) expression in cerebral cortex and hippocampus of chronic ischemic vascular dementia rats . Methods Thirty‐six healthy SD rats were divided into the control group ,sham operation group and model group ,12 cases in each group .The chronic ischemic vascular dementia rat model was established by the permanent bilateral carotid artery occlusion The sham operation group received the same treatment to the model group except without bilateral carotid artery occlusion .The learning and memory abilities were tested by the Morris water maze experiment and climbing rope strength experiment at 1 ,4 ,8 ,12 weeks respectively .The expressions of VEGF and HO‐1 in rat cerebral cortex and hippocampus was determined by immunohistochemical SP technique .Results The escape latency time at 8 ,12 weeks in the model group was longer than that in the sham operation group and control group ,and the number of crossing the platform was less than that in the sham operation group and control group ,the differences were statistically significant (P<0 .05);the time of climbing at 1 -12 weeks had no statistical difference between the model group and the sham operation group and between the model group and the control group (P>0 .05) .The positive expression of HO‐1 and VEGF protein contents in the control group and sham operation group was less than that in the model group with sta‐tistical difference(P<0 .05) .Conclusion Chronic cerebral hypoperfusion has a permanent damage to the learning and memory abil‐ities in rats ,while has no influence on the motor function .VEGF and HO‐1 may play a protective role in chronic cerebral ischemia .关键词
痴呆 ,血管性/血管内皮生长因子/血红素氧化酶-1/学习记忆Key words
dementia ,vascular/vascular endothelial growth factor/heme oxygenase-1/learning and memory分类
医药卫生引用本文复制引用
沈瑞乐,常文广,吴艳芝,滕军放..慢性缺血性痴呆大鼠大脑皮层及海马中 HO-1、VEGF表达的变化[J].重庆医学,2016,45(18):2475-2477,3.
