动物医学进展2016，Vol.37Issue(5)：5-9,10,6.
猪瘟病毒 E0蛋白的原核表达及多克隆抗体的制备
Prokaryotic Expression of E0 Protein of Classical Swine Fever Virus and Preparation of Its Polyclonal Antibodies
摘要
Abstract
To express E0 protein of CSFV and prepare its polyclonal antibodies (pAb),the recombinant E0 protein of CSFV Shimen strain was expressed in E.coli BL21(DE3)with IPTG induction and successively purified by affinity chromatography and gel slice.Balb/c mice were immunized four times with the purified recombinant E0 protein.SDS-PAGE and Western blot analysis showed that recombinant protein was ex-pressed mainly in the inclusion body.The indirect ELISA showed that the antibody titers in the serum of immunized mice was at 1:50000.Moreover,the obtained anti-E0 pAb can specifically react with the E0 pro-tein expressed in virus-infected cells and E0-EGFP-expressing cells.The obtained E0 protein and its poly-clonal antibodies in this study will be useful for future studies on the function and immunogenicity of CSFV E0 protein.关键词
猪瘟病毒/E0蛋白/原核表达/多克隆抗体Key words
Classical swine fever virus/prokaryotic expression/E0 protein/polyclonal antibody分类
农业科技引用本文复制引用
贾俊杰,张必凯,张莉,谢金鑫,郭焕成,龚文杰,涂长春..猪瘟病毒 E0蛋白的原核表达及多克隆抗体的制备[J].动物医学进展,2016,37(5):5-9,10,6.基金项目
中国博士后科学基金项目（2013M532129）；国家自然科学基金项目 （）
