动物医学进展2016，Vol.37Issue(5)：20-25,6.
沙门菌19个毒力岛标志性基因的 PCR 检测
PCR Detection of Iconic Genes in 19 Salmonella Pathogenicity Islands
摘要
Abstract
To develop a PCR method for detection of Salmonella pathogenicity island genes and to investi-gate the distribution of pathogenicity islands in Salmonella,by comparing selected 45 virulence genes as the iconic genes of 19 pathogenicity islands,PCR assay was established for detecting S.typhi,S.enteritidis,S. typhimurium and S.bergedorf.45 iconic virulence genes distributed among the 19 pathogenicity islands were validated in S.typhi,S.enteritidis,S.typhimurium and S.bergedorf.The length of amplified prod-ucts were the same as expected and bands of which were rather typical and clear.The detection rates of 45 virulence genes were 75.56%,75.56%,66.67% and 42.22%,respectively,in S.typhi,S.enteritidis,S. typhimurium and S.bergedorf.The PCR method which was accurate,rapid and low-cost was available for studying Salmonella pathogenicity islands and evaluating the relative risk.关键词
沙门菌/毒力岛/标志性基因/PCR 检测Key words
Salmonella/pathogenicity island/iconic gene/PCR detection分类
农业科技引用本文复制引用
胡兴娟,沈飚,王忠发,杨赛军,徐君辉..沙门菌19个毒力岛标志性基因的 PCR 检测[J].动物医学进展,2016,37(5):20-25,6.基金项目
国家质检总局科技计划项目（2014IK278）；浙江省分析测试科技计划项目 （）
