动物医学进展2016，Vol.37Issue(5)：42-46,5.
广东水禽源大肠埃希菌耐药性及氨基糖苷类耐药基因研究
Investigation on Aminoglycosides Antibiotics Resistance and Resistance Genes of Escherichia coli Isolated from Waterfowl in Guangdong Province
张济培 1韦庆兰 1谭华龙 2陈冈 1陈建红1
作者信息
- 1. 佛山科学技术学院生命科学学院，广东佛山 528231
- 2. 华南农业大学兽医学院，广东广州 510642
- 折叠
摘要
Abstract
In order to understand the resistance to aminoglycosides antibiotics and the prevalence of amin-oglycosides resistance genes from waterfowl in Guangdong province,explore the relationship between anti-biotic resistance genes and phenotypes of antimicrobial resistance,antimicrobial susceptibilities of total 251 E.coli isolates to aminoglycosides antimicrobial agents were determined by agar dilution methods and the aminoglycosides resistance genes were detected by PCR.The results of drug sensitivity tests showed that the resistant incidence rates of ducks and geese were exhibited to streptomycin (81.58% and 75.43%), gentamicin (25% and 38.86%),spectinomycin (21.05% and 74.29%),kanamycin (38.16% and 54.86%) and amikacin (1.32% and 30.28%).The occurrences of aadA1 and aph(3′)-1 genes were ranked the high-er (84.6%-91.9%).It indicated that the antimicrobial resistance genetypes of aadA1 and aph(3′)-1 were detected frequently among commensal E.coli from waterfowl in Guangdong province.Analysis of resistance genetypes and drug resistance phenotype correlations showed that rmtB gene had significant correlation on the resistance of E.coli to gentamicin,amikacin,kanamycin and spectinomycin,which indicated rmtB gene played an important role in the resistant strains of E.coli.This research played a significant role in preven-tion of colibacillosis,and provided relevant data for the resistance genes of E.coli.关键词
水禽大肠埃希菌/氨基糖苷类药物/耐药基因型/耐药表型Key words
Escherichia coli from waterfowl/aminoglycoside antibiotics/resistance gene/resistance gene-type分类
农业科技引用本文复制引用
张济培,韦庆兰,谭华龙,陈冈,陈建红..广东水禽源大肠埃希菌耐药性及氨基糖苷类耐药基因研究[J].动物医学进展,2016,37(5):42-46,5.
