动物营养学报2016，Vol.28Issue(5)：1462-1470,9.DOI:10.3969/j.issn.1006-267x.2016.05.022
益生菌抵御大鼠急性腹泻的效果及其对肠道黏膜屏障的影响
Effects of Probiotics to Against Acute Diarrhea and on Intestinal Mucosal Barrier of Rats
摘要
Abstract
The purpose of this study was to evaluate the effects of Clostridium butyricum or Lactobacillus sali⁃varius ZJ614 on prevention of acute diarrhea and protection of intestinal mucosal barrier function. Forty⁃eight 21⁃day⁃weanned Wistar rats with the average body weight of (57.45±6.23) g were selected and randomly di⁃vided into four groups:normal control group ( C1 group) , model control group ( C2 group) , Clostridium bu⁃tyricum group ( T1 group) and Lactobacillus salivarius ZJ614 group ( T2 group) . Each group had six repli⁃cates, and each replicate had two rats. This experiment lasted for 9 days. C1 and C2 groups were orally admin⁃istered saline, and T1 and T2 groups were administered probiotics accordingly. On the 8th day, every group except C1 group was administered folium senna to cause acute diarrhea and then record diarrhea condition of rats. All rats were sacrificed, and blood and intestinal samples were collected at the end of experiment. The re⁃sults showed as follows:1) all rats displayed diarrhea symptoms as long as administered folium senna. Loose stools rate, diarrhea index, serum endotoxin and D⁃lactic acid contents were significantly increased in C2 group compared with C1 group ( P<0.01) . The diarrhea index, serum endotoxin and D⁃lactic acid contents decreased significantly in T1 and T2 groups compared with C2 group ( P<0.01) . 2) Compared with C2 group, the crypt depth in T1 and T2 groups was significantly decreased (P<0.05), the ratio of villus height to crypt depth in T1 and T2 groups was significantly increased ( P<0.01) , and the relative expression levels of ileum zonula oc⁃cludens⁃1 ( ZO⁃1) ( P<0.01) and occludin mRNA ( P<0.05) in T1 group were significantly improved. 3) C2 group significantly up⁃regulated the relative expression levels of ileum interleukin⁃10 (IL⁃10) (P<0.01)and interleukin⁃1β( IL⁃1β) mRNA ( P<0.05) compared with C1 group, and T1 group down⁃regulated the relative expression level of IL⁃10 mRNA compared with C2 group ( P<0.01) . In summary, Clostridium butyricum or Lactobacillus salivarius ZJ614 can alleviate the actue diarrhea induced by senna on a certain degree, and also decrease the intestinal permeability, and enhance intestinal mucosal barrier.关键词
酪酸梭菌/唾液乳酸菌/大鼠/急性腹泻/肠道黏膜屏障Key words
Clostridium butyricum/Lactobacillus salivarius ZJ614/rats/actue diarrhea/intestinal mucosal barrier分类
农业科技引用本文复制引用
庞敏,卢庆萍,朱丽媛,夏冰,张宏福..益生菌抵御大鼠急性腹泻的效果及其对肠道黏膜屏障的影响[J].动物营养学报,2016,28(5):1462-1470,9.基金项目
国家科技支撑项目（2012BAD39B01）；中国农业科学院创新团队 （）
