解放军医药杂志2016，Vol.28Issue(6)：9-13,5.
模拟海拔8000m高原缺氧环境对大鼠脑组织线粒体自噬的影响
Effect of Simulated 8000 m Altitude on Mitochondrial Autophagy of Brain Tissues in Rats under Hypobaric Hy-poxia Environment
摘要
Abstract
Objective To study the effect of simulated 8000 m altitude using large scale of low pressure oxygen cabin on mitochondrial autophagy of brain tissues in rats under hypobaric hypoxia environment. Methods A total of 30 Wistar rats were randomly divided into normal group and 6 h,12 h,24 h and 48 h hypoxia groups( n = 6 for each group). Under simulated 8000 m altitude using large scale of low pressure oxygen cabin,brain tissues were collected and observed using transmission electron microscopy(TEM),and mitochondrial autophagy protein light-chain 3(LC3),Be-clin1 and apoptosis related proteins of cytochrome C(CytC),Caspase3 and Bcl-2 related X(Bax)were detected. Re-sults The TEM result showed that more cavitation and intercellular space,the part injury of cells membrane,cells dam-age and higher degrees of autophagosomes incidence in brain tissues of different times of hypoxia groups were found,com-pared with those of normal group,and the most values of autophagosome degree and amount were found in 24 h hypoxia group. Levels of apoptosis related proteins CytC,Caspase3 and Bax expressions were increased with prolonged hypoxia time in different times of hypoxia groups,and the levels of 24 h and 48 h hypoxia groups were significantly higher than those of normal group(P ﹤ 0. 01). Autophagy related protein LC3 and Beclin1 showed a trend from increasing to decrea-sing with prolonged hypoxia time,and the highest LC3 level was found in 24 h hypoxia group(P ﹤ 0. 05);Beclin1 pro-tein level began to increase in 6 h hypoxia group,and the highest Beclin1 level was found in 12 h hypoxia group( P ﹤0. 01). Conclusion Simulated 8000 m anoxic environment can induce damage in rats,promote expressions of apoptosis related proteins and incidence of mitochondrial autophagy in brain tissues at the same time.关键词
缺氧,脑/线粒体/自噬/高原病/大鼠,WistarKey words
Hypoxia,brain/Mitochondria/Autophagy/Altitude sickness/Rats,Wistar分类
医药卫生引用本文复制引用
蒙萍,王宁,漆欣柱,王昕,何蕾,马慧萍..模拟海拔8000m高原缺氧环境对大鼠脑组织线粒体自噬的影响[J].解放军医药杂志,2016,28(6):9-13,5.基金项目
国家自然科学基金面上项目（81571847、81402848）；甘肃省自然科学基金项目（145RJZA089）；全军医药卫生科研基金项目 （）
