计量学报2016，Vol.37Issue(3)：328-332,5.DOI:10.3969/j.issn.1000-1158.2016.03.22
人血清白蛋白纯品含量的同位素稀释质谱方法研究
Determination of the Mass FraCtion of Pure Human Serum Albumin by HPLC-lDMS
李佳乐 1武利庆 2金有训 3王晶 3杨彬 3杨屹3
作者信息
- 1. 北京化工大学，北京 100029
- 2. 中国计量科学研究院，北京 100029
- 3. 中国计量科学研究院，北京 100029
- 折叠
摘要
Abstract
A mass balance method and a high performance liquid chromatography-isotope dilution mass spectrometry (HPLC-IDMS)method are established to determine the mass fraction of pure human serum albumin(HSA). With the mass balance method,the pure HSA is quantified by HPLC and its moisture and ash are quantified by Karl Fischer and ignition residue analysis,which give an average mass fraction of 0. 861 g/g. By the HPLC-IDMS method,after the pure HSA is hydrolyzed by acid,the resulted Pro,Val and Phe are quantified. Consequently,the mass fraction of HSA is calculated by the average. Under the optimized conditions,the mass fraction of HSA is 0. 853 g/g with a relative standard deviation(RSD)of 0. 8% and an expanded uncertainty of 0. 015 g/g(k = 2). The limits of detection and quantification are 1. 37 í 10 - 5 g/g and 4. 55 í 10 - 5 g/g,respectively. Compared with the mass balance method,the relative bias of the HPLC-IDMS method is 0. 9% .关键词
计量学/人血清白蛋白/定量/同位素稀释质谱法/质量平衡法Key words
metrology/human serum albumin/mass fraction/high performance liquid chromatography-isotope dilution mass spectrometry/mass balance method分类
通用工业技术引用本文复制引用
李佳乐,武利庆,金有训,王晶,杨彬,杨屹..人血清白蛋白纯品含量的同位素稀释质谱方法研究[J].计量学报,2016,37(3):328-332,5.
