内蒙古民族大学学报（自然科学版）2016，Vol.31Issue(3)：236-239,243,5.DOI:10.14045/j.cnki.15-1220.2016.03.014
斑马鱼胚胎检测狼毒不同炮制方法毒性
Toxicity Detection of Stellera Chamaejasme in Zebrafish Embryo with DifferentExtraction Methods
摘要
Abstract
As a perennial herb used in Mongolian medicine, Sellera chamaejasme has strong anticancer effects but is also highly toxic. To reduce its toxicity, the effects of alcohol (A), milk(M1), yogurt(M2), vinegar(S) and myrobalan(K) processing on zebra embryos were compared. Vinegar extraction was the most toxic. Even at 500 ug/ml and 50 ug/ml, 55%and 32.5%of zebrafish embryos were killed within 48 h. The mortality rate remains almost the same by other ex-tracting methods. Exposure to all the extractions results in rugged appearance of caudal fins and cells of that region shows positive staining with TUNEL. The number of apoptotic cells of zebrafish embryo tail fins increased from 3.3 in the control group to 9.6-18.3 in the exposure group. A further study will be made on proliferation and apoptosis re-lated gene and protein expression.关键词
斑马鱼胚胎/毒性/细胞凋亡/炮制/狼毒Key words
Zebrafish embryos/Toxicity/Cell apoptosis/Processing/Stellera Chamaejasme分类
医药卫生引用本文复制引用
杨景峰,董文静,宗俊成,李明,邰塞击拉呼,许良,杨斌,董武..斑马鱼胚胎检测狼毒不同炮制方法毒性[J].内蒙古民族大学学报（自然科学版）,2016,31(3):236-239,243,5.基金项目
国家自然科学基金 （）
内蒙古自治区自然科学基金（Nos.2012MS1203,2015MS0804）；草原英才工程项目（2015-2016）；内蒙古自治区高等学校科学研究项目（NJZY11202）；内蒙古民族大学科学研究项目 （）
内蒙古自治区科技创新引导项目（2015-2016）；人才开发基金项目 （）
