农药学学报2016，Vol.18Issue(3)：304-310,7.DOI:10.16801/j.issn.1008-7303.2016.0041
耿氏硬草对乙酰辅酶A羧化酶类除草剂抗性水平及分子机制初探
Resistance to acetyl-CoA carboxylase-inhibiting herbicides in Pseudosclerochloa kengiana and its molecular resistance mechanism
摘要
Abstract
Whole-plant dose-response experiments were conducted to investigate the resistance levels of the putative resistant Pseudosclerochloa kengiana population to acetyl-CoA carboxylase(ACCase)-inhibiting herbicides. And ACCase gene fragments were amplified, sequenced and compared with the susceptible population, so as to identify its molecular resistance mechanism. The results of whole-plant dose-response assays showed that the putative resistant population SD-32 was resistant to fenoxaprop-p-ethyl (16.5-fold), clodinafop-propargyl (7.5-fold), sethoxydim (15.0-fold), clethodim (4.4-fold), and pinoxaden (5.7-fold) compared to the reference susceptible population SD-6. Partial chloroplastic ACCase sequences revealed that the resistant population SD-32 possessed an Asp-to-Gly substitution at the ACCase codon position 2078, which resulted from a nucleotide change from GAT to GGT. This study concluded that the change from Asp to Gly mutation at the amino acid position 2078 in the plastid ACCase was an important reason for the resistance to ACCase-inhibiting herbicides in P. kengiana.关键词
耿氏硬草/乙酰辅酶A羧化酶/除草剂/抗性水平/基因突变Key words
Pseudosclerochloa kengiana/acetyl-CoA carboxylase/herbicide/resistance level/mutation分类
农业科技引用本文复制引用
袁国徽,王恒智,赵宁,路兴涛,刘伟堂,王金信..耿氏硬草对乙酰辅酶A羧化酶类除草剂抗性水平及分子机制初探[J].农药学学报,2016,18(3):304-310,7.基金项目
国家公益性行业(农业)科研专项(201303031)；国家自然科学基金(31471787) （农业）
