农药学学报2016，Vol.18Issue(3)：323-329,7.DOI:10.16801/j.issn.1008-7303.2016.0044
草铵膦对斑马鱼胚胎及仔鱼的毒性研究
Toxic effects of glufosinate-ammonium on zebrafish embryos and larvae
摘要
Abstract
Glufosinate-ammonium (GLA) is a broad spectrum non-selective herbicide. The increasing usage of GLA has drawn people's attention to questions of its biological safety. In this study, zebrafish embryos (Danio rerio) were used as a laboratory model to test concentration dependent responses of GLA including mortality and morphological changes. In addition, in situ hybridization was employed to investigate the effect on Vasa gene expression which is known to be important in reproduction. And QRT-PCR was used for the quantification. The results revealed that embryo mortality was increased after the exposure to 1.6 μg/L GLA. And the induced curvature of tail, and the inhibition of pigmentation were observed after exposing to 1.3 μg/L GLA. Furthermore, Vasa expression missing was also induced by GLA (1.3 μg/L) during the in situ hybridization, and the Vasa gene expression was decreased by 3.8 fold compared to the control group. The above results indicate that GLA has lethal toxicity, teratogenicity and potential of reproductive toxicity.关键词
草铵膦/斑马鱼/毒性评价/Vasa基因Key words
glufosinate-ammonium (GLA)/zebrafish/toxicity assessment/Vasa gene分类
化学化工引用本文复制引用
王丰,董武,陈形,杨达汉,杨景峰,康桂英,赵宝全,李小满,蒲韵竹,郑娜..草铵膦对斑马鱼胚胎及仔鱼的毒性研究[J].农药学学报,2016,18(3):323-329,7.基金项目
国家自然科学基金(30360090,21267015,81360508,21567019)；内蒙古自治区自然科学基金(2012MS1203)；内蒙古自治区人才开发基金；内蒙古自治区“草原英才”工程(2014)；内蒙古自治区高等学校科学研究项目(NJZY11202) （30360090,21267015,81360508,21567019）
