农药学学报2016，Vol.18Issue(3)：337-343,7.DOI:10.16801/j.issn.1008-7303.2016.0046
啶虫脒金标免疫速测试纸条研制及其在茶叶中的应用
Development and application of gold immunostrip for the detection of acetamiprid residue in tea samples
摘要
Abstract
The development of a gold immunostrip for the rapid detection of acetamiprid residue in tea samples was described. It was based on the principle of direct competitive immunochromatography. The artificial antigen of acetamiprid was prepared for immunization, and a highly-sensitive monoclonal antibody was obtained with the titer of 1:10 000. Subsequently, the gold immunostrip was developed and the visual detection limit of acetamiprid was 10 ng/mL. The detection time was just 10 min. No cross reaction with other neonicotinoid pesticides, such as imidacloprid, thiamethoxam, and nitenpyram, was detected. Moreover, this method has been used to determine the acetamiprid residue in tea (green tea, black tea and extra-strong tea) by spiked recovery tests. And results indicated that this method could meet the detection requirement of Chinese MRL of acetamiprid (0.5 mg/kg) in tea. Also, it possessed some advantages such as high efficiency, convenience, and low cost. Overall, the newly-developed immunostrip can be used in field tests for rapid screening of fresh tea before tea harvest and the quality control of tea products.关键词
啶虫脒/残留/抗体/金标试纸/茶叶/快速检测Key words
acetamiprid/residue/monoclonal antibody/gold immunostrip/tea/rapid screening分类
农业科技引用本文复制引用
赵颖,杨斌,柳颖,方一画,司芳芳,郭逸蓉,程敬丽,朱国念..啶虫脒金标免疫速测试纸条研制及其在茶叶中的应用[J].农药学学报,2016,18(3):337-343,7.基金项目
公益性行业(农业)科研专项经费项目(201203094-3)；国家自然科学基金项目(31401768)；浙江大学实验技术研究项目(SZD201404) （农业）
