农药学学报2016，Vol.18Issue(3)：373-379,7.DOI:10.16801/j.issn.1008-7303.2016.0051
超高效液相色谱法检测啶虫脒在工厂化双孢蘑菇栽培中的残留
Determination of acetamiprid residue in industrial cultivation of Agaricus bisporus by ultra performance liquid chromatography
摘要
Abstract
The transformation of acetamiprid during Agaricus bisporus industrial production was investigated by testing its residues in cover-soil, compost and fruits using ultra performance liquid chromatography (UPLC-PDA). The samples were extracted with acetonitrile and purified by dispersive solid phase. Acetamiprid residue was then determined by UPLC-PDA. At 0.02-3 mg/kg spiked levels, the average recoveries for cover-soil, compost and fruits were from 81% to 97% (RSDs ≤9.6%). In different application levels, the dissipation of acetamprid in soil and compost was found to conform to the first order kinetics equation and the average half-life was 34 d in cover-soil and 23 d in compost. The acetamprid applied in cover-soil moved into compost. And residues in the compost increased in the first 21-28 d and then started to decrease. At the application rate of 10-250 mg a.i./kg in soil or compost, acetamprid residues in fresh mushroom fruits were 0.03-2.6 mg/kg. And there were significant residue differences among the first, second and third harvesting time stages. At the same pesticide concentration, the acetamiprid residue level in fruit bodies is significantly higher when the pesticide was applied in the cover-soil compared to that when applied in the compost.关键词
超高效液相色谱/啶虫脒/双孢蘑菇/消解/残留Key words
ultra performance liquid chromatography/acetamiprid/Agaricus bisporus/dissipation/residue分类
农业科技引用本文复制引用
张其才,董茂锋,宋卫国,付白莲,王伟民,赵志辉,王坦,陈珊珊,曲明清..超高效液相色谱法检测啶虫脒在工厂化双孢蘑菇栽培中的残留[J].农药学学报,2016,18(3):373-379,7.基金项目
国家自然科学基金项目(31301699)；公益性行业(农业)科研专项(201303088) （31301699）
