农药学学报2016，Vol.18Issue(3)：393-396,4.DOI:10.16801/j.issn.1008-7303.2016.0054
雷帕霉素对4种植物病原真菌的抑菌活性
Antifungal activity of rapamycin against four kinds of plant pathogenic fungi
摘要
Abstract
Rapamycin is an important marocyclic polyketide produced by Streptomyces hygroscopicus, which exhibited immunosuppressive and antifungal properties. In this study, antifungal activity of rapamycin against four kinds of plant pathogens, including Botrytis cinerea, Sclerotinia sclerotiorum, Rhizoctonia solani, and Fusarium oxysporum, was assayed by the mycelium growth rate method. The results showed that rapamycin exhibited extreme significant inhibit efficacy against mycelial growth of all of these plant pahogens on the PDA medium. The EC50 value of rapamycin against S. sclerotiorum was 2.23 × 10–4 μg/mL, which is the lowest. The EC50 values against B. cinerea, R. solani, and F. oxysporum were 1.32 × 10–3, 4.05 × 10–3 and 3.82 × 10–3 μg/mL, respectively. It is noteworthy that the toxicity of rapamycin against the mycelial growth of B. cinerea is significantly higher than those of azoxystrobin and propamidine, with the EC50 values of 3.24 and 3.81 μg/mL, respectively. The hypha of B. cinerea exhibited premature aging after treated with rapamycin under the scanning electron microscope. The results in this study provided the basis for investigating the efficient inhibitation mechanism of rapamycin in the future study.关键词
雷帕霉素/抑菌活性/植物病原真菌/番茄灰霉病菌Key words
rapamycin/antifungal activity/plant pathogenic fungi/Botrytis cinerea分类
农业科技引用本文复制引用
王彦,徐曦,张立钦,王勇军..雷帕霉素对4种植物病原真菌的抑菌活性[J].农药学学报,2016,18(3):393-396,4.基金项目
公益性行业(农业)科研专项(201303106)；浙江省自然科学基金(LY12C14006)。 （农业）
