山东医药2016，Vol.56Issue(24)：5-8,4.DOI:10.3969/j.issn.1002-266X.2016.24.002
胃癌组织 ATP1 A2的表达及机制探讨
Expression of ATP1A2 in gastric cancer and its mechanism
摘要
Abstract
Objective To observe the expression of ATP1A2 in gastric cancer tissues and to investigate the correla-tions of ATP1A2 expression with focal adhesion , cell adhesion molecules , calcium signaling and ECM receptor interaction pathways.Methods TCGA dataset which included 293 cases, and GEO dataset GSE62254 which included 300 cases, were collected.ATP1A2 expression was trisected into 3 levels: low, median, and high.The cutoff was set to 0.33 and 0.67.The relationships between ATP1A2 and clinicopathological characteristics as well as prognosis were assessed .GSEA was used to predict the functional gene sets modulated by ATP 1A2.Results There were 95 cases of low expression , 99 of median expression and 99 of high expression in TCGA dataset .The expression of ATP1A2 was significantly associated with T stage, lymph node metastasis, TNM stage and differentiation (all P<0.01), but was not associated with gender and age (all P>0.05).The median survival durations were 70, 36.9, and 26 months in low, median, and high ATP1A2 expres-sion subgroups, respectively.The P value was less than 0.05 in the log-ranked test.While in GSE 62254 dataset, there were 99 low expressed, 102 median expressed and 99 high expressed cases.Different expression of ATP1A2 was signifi-cantly associated with gender , age, T stage, N stage, pTNM stage and Lauren types (all P<0.05).The median survival duration in the high ATP1A2 subgroup was 31 months, while it was much longer in the median and low expression sub-groups (P<0.01).In GSEA, focal adhesion, cell adhesion molecules, calcium signaling, and extracellular matrix recep-tor interaction pathways were enriched in samples with high ATP 1A2 expression (P<0.05 or P<0.01).Conclusions ATP1A2 is highly expressed in gastric cancer tissues and the high expression indicates poor prognosis .The underlying mechanism might be that the high expression of ATP 1A2 causes the abnormity of focal adhesion , cell adhesion molecules , calcium signaling and extracellular matrix receptor interaction pathways .关键词
胃癌/癌症基因组图谱/人类肿瘤相关基因表达汇编/ATP酶α2亚基Key words
gastric carcinoma/cancer genome atlas/human cancer-related gene expression assembly/ATP1A2分类
医药卫生引用本文复制引用
李智,王硕,侯科佐,臧丹,郑春雷,车晓芳,曲秀娟,刘云鹏..胃癌组织 ATP1 A2的表达及机制探讨[J].山东医药,2016,56(24):5-8,4.基金项目
国家自然科学基金资助项目（81302023）；“重大新药创制”科技重大专项课题（2013ZX09303002）；辽宁省科学技术计划项目（2014225013）。 （）
