生物技术通报2016，Vol.32Issue(6)：69-75,7.DOI:10.13560/j.cnki.biotech.bull.1985.2016.06.011
转Bt cry1Ah基因抗虫玉米对亚洲玉米螟、棉铃虫和黏虫的抗性评价
Resistance Evaluation of Btcry1Ah-transgenic Maize to Asian Corn Borer,Cotton Bollworm and Oriental Armyworm
摘要
Abstract
Bt cry1Ah gene was transferred into inbred line maize Zong31 via Agrobacterium-mediated method,and transgenic maize HGK60 with significant resistance to corn borer was acquired. In order to investigate its insecticidal activity to Lepidoptera pests,we evaluated the insecticidal effects of HGK60 to Asian corn boner(Ostrinia furnacalis),cotton bollworm(Mythimna separate(Walker))and oriental armyworm(Helicoverpa armigeraHubne)through laboratory and field bioassay. The results of laboratory bioassay indicated that no Asian corn boner feeding on HGK60 leaves survived. HGK60 presented the toxic effect on neonate of cotton bollworm,and different tissues of it had different insecticidal effects. Compared to the non-transgenic maize,the body-weight of armyworm neonate was significantly inhibited after a week of feeding HGK 60 leaves. The results of field bioassay showed that HGK60 had solid insecticidal effects to O. furnacalis and H. armigera in high resistance level,while the efficacy to M. separate was in resistance level.关键词
cry1Ah基因/玉米/玉米螟/棉铃虫/黏虫Key words
cry1Ah gene/maize/Ostrinia furnacalis/Helicoverpa armigera/Mythimna separate Hubne引用本文复制引用
宋苗,汪海,张杰,何康来,梁革梅,朱莉,黄大昉,郎志宏..转Bt cry1Ah基因抗虫玉米对亚洲玉米螟、棉铃虫和黏虫的抗性评价[J].生物技术通报,2016,32(6):69-75,7.基金项目
转基因生物新品种培育重大专项 （）
