生物技术通报2016，Vol.32Issue(6)：193-198,6.DOI:10.13560/j.cnki.biotech.bull.1985.2016.06.028
海洋细菌L1-9双抗菌株的定殖能力及其对黄瓜枯萎病的防治作用
Colonization of Double-resistance Strain of Marine Bacterium L1-9 and Its Biocontrol Effect on Fusarium Wilt of Cucumber
摘要
Abstract
The Paenibacillus polymyxastrain L1-9 of marine bacterium was labeled by antibiotic marker,and strain L1-9Str,rif showed the resistance to rifampicin and streptomycin at the concentrations of 160 μg/mL and 20 μg/mL,respectively. The antibacterial characters of double-resistance strain L1-9Str,rif and its resistance to rifampicin and streptomycin were still stable after 10 times subculturing. The pot experiment showed that L1-9Str,rif successfully colonized in cucumber rhizosphere soil,root tissue,stem base,cotyledon,and true leaves. The colonized dynamics of L1-9Str,rif in cucumber ecto-rhizosphere soil,rhizosphere and rhizoplane soil,and cucumber tissues were similar;the amount of L1-9Str,rif colonized in cucumber tissue was little in the early stage,but increased gradually along with the cucumber growth,and reached a peak,then decreased gradually. The amount of L1-9Str,rif was the most in rhizoplane soil(1.76×109 CFU/g),the followed in the rhizosphere, while the least in ecto-rhizosphere soil. The detection of L1-9Str,rif in cucumber tissues showed that it was the most in cotyledons(5.63×104 CFU/g), followed by its number in roots and stem bases(0 - 2 cm). The amount of bacteria in the rhizosphere soil was still in a stable level at 26 d, and the quantity from rhizoplane soil was the highest with 2.41×107 CFU/g. The quantity of bacteria in the cotyledons got the highest of 4.15×104 CFU/g among cucumber tissue samples. The greenhouse test showed that both strain L1-9 and strain L1-9Str,rif had favorable control effects on fusarium wilt of cucumber with the control efficacy over 70% at different stages. The above results indicated that strain P. polymyxastrain L1-9 may colonize in cucumber rhizosphere soil and tissues,and present a great potential for the bio-control of fusarium wilt of cucumber.关键词
海洋细菌/多黏类芽孢杆菌/抗生素标记/黄瓜枯萎病/定殖Key words
marine bacteria/Paenibacillus polymyxa/antibiotic marker/fusarium wilt of cucumber/colonization引用本文复制引用
王军强,汪晶晶,王琦,马桂珍,暴增海,王淑芳,周向红..海洋细菌L1-9双抗菌株的定殖能力及其对黄瓜枯萎病的防治作用[J].生物技术通报,2016,32(6):193-198,6.基金项目
江苏省自然科学基金项目（BK20141248），江苏省高校自然科学重大项目（12KJA210001），连云港市科技局农业攻关项目 （CN1307），国家级大学生实践创新训练计划项目（G201411641105002），江苏省“十二五”高等学校水产类重点专业项目资助 （）
