生物技术通报2016，Vol.32Issue(6)：205-210,6.DOI:10.13560/j.cnki.biotech.bull.1985.2016.06.030
土壤中木霉的分离及其对毒死蜱降解特性研究
Isolation and Characterization of the Chlorpyrifos-degrading Trichoderma Strains from the Vegetable Soil in Greenhouse
摘要
Abstract
To explore and protect the Trichoderma resources that can degrade chlorpyrifos,six different types of Trichoderma strains with high activity of degrading chlorpyrifos were screened from the long-term organophosphorus pesticide contaminated soil. Analyses of morphological characteristics combined with internal transcribed spacer(ITS)rDNA sequences were used to identify them as T. harzianum, T. viride,T. koningii,T. koningiopsis,T. longibrachiatum,and T. brevicompactum,respectively. T. longibrachiatum TC5 with the highest degradation activity was selected to investigate the characterization of degrading chlorpyrifos under the culture condition including extra carbon source,pesticide concentration,inoculum density and pH. Under neutral or alkaline conditions,the strains TC5 had solid degradation activity to chlorpyrifos. Either adding carbon or increasing the inoculum concentration promoted the degradation rate. Degradation activity gradually increased with the increasing of chlorpyrifos concentration in the range of 50-300 mg/L. In the pot experiment,Trichoderma TC5 remained the high degradation activity to chlorpyrifos,but the degradation activity in the natural soil was significantly lower than in sterilized soil. The Trichoderma strains have great potential application in remedying the pesticide-contaminated soil.关键词
木霉/毒死蜱/生物降解Key words
Trichoderma/chlorpyrifos/biodegradation引用本文复制引用
张广志,张新建,李红梅,郭凯,杨合同..土壤中木霉的分离及其对毒死蜱降解特性研究[J].生物技术通报,2016,32(6):205-210,6.基金项目
山东省科技发展计划（2014GSF121028，2013GNC11019），国家科技基础性工作专项 （）
