天津科技大学学报2016，Vol.31Issue(3)：11-15,5.DOI:10.13364/j.issn.1672-6510.20150071
热休克蛋白质DnaK的纯化及其二聚体性质研究
Purification of DnaK Mutants and the Characteristics of DnaK Dimer
摘要
Abstract
Two DnaK mutants were constructed by site-directed mutagenesis to study the effect of ATP or ADP on heat shocked protein.DnaK-A303C and DnaK-H541C were expression through induction.Then the recombined proteinswere purified by using sulfate nickel affinity chromatography and anion exchange chromatography.The same amount of proteins was oxidized and the protein cross-linking was detected by SDS-PAGE.The results show that DnaK mutants formed dimmer in the presence of ADP.However,the heterodimer was found under the condition of ATP.This study provides an experimen-tal basis for understanding the synergism between the two subunits of heat shocked protein.关键词
DnaK/ATP/二聚体/突变体Key words
DnaK/ATP/dimer/mutant分类
轻工纺织引用本文复制引用
刘清岱,苏家跃,田雪利,杨营,赵飞,盛长忠..热休克蛋白质DnaK的纯化及其二聚体性质研究[J].天津科技大学学报,2016,31(3):11-15,5.基金项目
国家自然科学基金资助项目(31571029)；天津市应用基础与前沿技术研究计划资助项目(15JCYBJC30300，15JCQNJC46300，15JCTPJC55400) （31571029）
