天津体育学院学报2016，Vol.31Issue(2)：141-146,173,7.DOI:10.13297/j.cnki.issn1005-0000.2016.02.009
连续跳跃条件下兔髌骨髌腱结合部组织形态学及相关生长因子表达特征
Characteristics of Patella-Patellar Tendon Junction in Histomorphology and Expression of Related Growth Fac-tors under the Condition of Continuous jumping
摘要
Abstract
Objective:To investigate the morphologic features and expression of related growth factor of Patella-Patellar Tendon Junction(PPTJ)injury caused by continuous jumping. Methods:Eighteen weeks old female New Zealand white rabbits were randomly divided into JE groups(JE)and control group (Con),with 5 rabbits in each group. Jumping scheme was 150 times a day,5 days a week. Patella-patellar tendon complex was harvested 4 weeks later,par⁃affin section were stained by HE and Safranin O to observe the histopathologic changes ,Immunohistochemistry was used to display the expression of related growth factors. Results:Compared with Con group,Cell density and Fibrocartilage zone thickness and Glycosaminoglycan area was significantly increased. Cell density in PPTJ inner zone was lower than that in PPTJ outer zone(P<0.05),whereas Fibrocartilage zone thickness in PPTJ inner zone was larger than that of PPTJ outer zone(P<0.05). The expression of CollagenⅢ,HIF-1α,VEGF and BMP-2 in PPTJ zone of JE group was higher than that of Con group. The expression of CollagenⅢin PPTJ outer zone of JE group was higher than that of PPTJ inner zone(P<0.05),while HIF-1αand VEGF was higher(P<0.01). Conclusion:Continuous jumping lead to PPTJ overuse injury,Pathological changes in PPTJ inner zone was different from that of PPTJ outer zone. The expression of CollagenⅢ,HIF-1αand VEGF in PPTJ outer zone was higher than that of PPTJ inner zone.关键词
髌骨髌腱结合部/过度使用性损伤/组织形态学/生长因子/髌腱腱病Key words
patella-patellar tendon junction/overuse injury/histomorphology/growth factor/patellar tendinopathy分类
社会科学引用本文复制引用
江大雷..连续跳跃条件下兔髌骨髌腱结合部组织形态学及相关生长因子表达特征[J].天津体育学院学报,2016,31(2):141-146,173,7.基金项目
中央高校基本科研业务费专项资助课题（项目编号2014BS015）；河南师范大学博士启动课题资助 （）
