中国组织工程研究2016，Vol.20Issue(23)：3349-3356,8.DOI:10.3969/j.issn.2095-4344.2016.23.001
miR-34a通过Notch1信号通路对肺癌干细胞的抑制
Inhibitory effect of miR-34a on lungcancerstem cellsvia Notch1 signaling pathway
摘要
Abstract
BACKGROUND:It has been proved that miR-34a plays an inhibitory role in the growth of lung cancer stem cels, but the underlying mechanism remains unclear. <br> OBJECTIVE:To explore the inhibitory effect of miR-34a on lung cancer stem celsand the underlying mechanism. <br> METHODS:The CD133+lung cancer stem cels were separated from lung cancer A549 cel lines using magnetic activated cel sorting method. And miR-34a-overexpressing CD133+lung cancer stem cels were established by liposome transfection technology. Besides,the targeted relationship between miR-34a and Notch1 was analyzed by the dual-luciferase reporter. Afterwards, Notch1 silencing was performed by gene knockout, and its effect on lung cancer stem cels was determined. <br> RESULTS AND CONCLUSION:After sorted and detected by immunomagetic selection and flow cytometry assay respectively, a high rate of CD133+lung cancer stem cel was obtained. And qRT-PCR detected that the expression level of miR-34a in CD133+lung cancer stem cels was significantly lower than that in CD133-lung cancer stem cels. Moreover, miR-34a-overexpressing CD133+lung cancer stem cels were successfuly constructedandmiR-34a significantly inhibited proliferation and induced apoptosis of lung cancer stemcels. Dual-luciferase reporter assay indicated that Notch1 mRNA was a target of miR-34a. In addition, Notch1 silencing obviously inhibited proliferation and induced apoptosis of lung cancer stem cels. These findings suggest that miR-34a can inhibite lungcancer stem celsviathe Notch1 signaling pathway.关键词
干细胞/肿瘤干细胞/肺癌干细胞/miRNA/miR-34a/Notch1信号通路/Notch1/肺腺癌A549细胞/CD133+肺癌干细胞/增殖/凋亡分类
医药卫生引用本文复制引用
韩纪昌,张祎捷,李红兵,杨存葆,马超楠,戚冠斌..miR-34a通过Notch1信号通路对肺癌干细胞的抑制[J].中国组织工程研究,2016,20(23):3349-3356,8.基金项目
河南省卫生厅中青年科技创新人才工程(4189号)@@@@the Science and Technology Innovation Project of Henan Provincial Health Department for Young Talents, No.4189 （4189号）
