中国组织工程研究2016，Vol.20Issue(23)：3464-3469,6.DOI:10.3969/j.issn.2095-4344.2016.23.018
过表达miR-32削弱多氯联苯抑制P19细胞向心肌细胞的分化
Overexpression of microRNA-32 weakens the inhibition of polychlorinated biphenyls to the differentiation of P19 cells into cardiomyocytes
张松雨 1李燕 1刘江波 1李纲1
作者信息
- 1. 郑州大学附属南阳医院，南阳市中心医院心内科，河南省南阳市 473009
- 折叠
摘要
Abstract
BACKGROUND:Polychlorinated biphenyls inhibit the differentiation of P19 cels into cardiomyocytes. In the meanwhile, microRNAs play an important role in regulating cel differentiation. <br> OBJECTIVE:To explore the effect of microRNA-32(miR-32) on the polychlorinated biphenyls-inhibited differentiation of P19 cels into cardiomyocytes. <br> METHODS:P19 cels were co-cultured with 2.5 μmol/L polychlorinated biphenyls and 1% dimethyl sulfoxide. Afterwards, α-actinin, desmin, cTnI and GATA4 were identified by western blot assay.Wddwxrof polychlorinated biphenyls on the expression of miR-32 was evaluated by real-time PCR. Mouse eukaryotic vector expressing miR-32 was constructed by gene recombination technology,andtransfected into P19 celsfolowed byco-culturedwith2.5 μmol/Lpolychlorinated biphenylsand1% dimethyl sulfoxide. Then, expressions of differentiation-related proteins were detected by western blot assay. <br> RESULTS AND CONCLUSION:Polychlorinated biphenylsinhibited the differentiation of P19 cels into cardio myocytes anddecreased the miR-32 expression. Cel lines overexpressing miR-32 was successfuly established in mice.Furthermore, we found thatoverexpression of miR-32 weakens the inhibition of polychlorinated biphenyls to the differentiation of P19 cels into cardiomyocytes.关键词
干细胞/分化/多氯联苯/miR-32/P19细胞/心肌细胞/过表达分类
医药卫生引用本文复制引用
张松雨,李燕,刘江波,李纲..过表达miR-32削弱多氯联苯抑制P19细胞向心肌细胞的分化[J].中国组织工程研究,2016,20(23):3464-3469,6.
