中国组织工程研究2016，Vol.20Issue(24)：3581-3587,7.DOI:10.3969/j.issn.2095-4344.2016.24.013
骨形态发生蛋白2/Osterix信号通路调控的前成骨细胞分化
Bonemorphogeneticproteins-2/Osterix signaling pathway regulates the differentiation of preosteoblasts
李成琳 1陈书兰 2任伟伟3
作者信息
- 1. 潍坊医学院口腔医学院，山东省潍坊市 261000
- 2. 青岛市市立医院口腔医学中心，山东省青岛市266071
- 3. 湖北医药学院附属东风口腔医院儿童口腔科，湖北省十堰市 442000
- 折叠
摘要
Abstract
BACKGROUND:Bone formation and development are reported to be regulated by bone morphogenetic protein2(BMP2)-induced Osterix expression. OBJECTIVE:To investigate the regulatory effect of BMP2/Osterix signaling pathway on differentiation of preosteoblasts in mice. METHODS:mRNA and protein expression of Osterix wasdetermined by real-time RT-PCR and western blot assay, respectively at various time points after mouse preosteoblasts were treated with BMP2. pcDNA3.1/myc-Osterix eukaryotic expression vector was constructed and transducted into preosteoblasts, and then mRNA expression of alkaline phosphatase, bone sialoprotein, and matrix extracelular phosphoglycoprotein wasdetected by real-time RT-PCR after transduction and BMP2 treatment. RESULTSANDCONCLUSION:Osterix mRNA expression was up-regulated when treated with BMP2 in mouse preosteoblasts, and reached the peak at 24 hours. In addition, the protein expression of Osterix was increased after BMP2 treatment. Alkaline phosphatase, bone sialoprotein, and matrix extracelular phosphoglycoprotein mRNA expression wasup-regulated after transfection of mouse preosteoblasts with pcDNA3.1/myc-Osterix eukaryotic expression vector and BMP2 treatment. Our results indicate that BMP2 regulates the synthesis of genetic markers of osteogenesis,such asalkaline phosphatase,matrix extracelular phosphoglycoproteinviaup-regulating Osterix expression in mouse preosteoblasts, suggesting BMP2/Osterix signaling pathway plays a critical role in bone development.关键词
组织构建/骨细胞/骨形态发生蛋白2/Osterix/前成骨细胞/实时定量RT-PCR/信号通路/免疫印迹分类
医药卫生引用本文复制引用
李成琳,陈书兰,任伟伟..骨形态发生蛋白2/Osterix信号通路调控的前成骨细胞分化[J].中国组织工程研究,2016,20(24):3581-3587,7.
