中国麻业科学2016，Vol.38Issue(6)：249-257,267,10.
利用SRAP和SSR标记构建红麻遗传连锁图谱
Constructing a Genetic Linkage Map by SRAP and SSR Marker for Kenaf(Hibiscus cannabinus L.)
摘要
Abstract
Construction of genetic linkage map for kenaf will lay good foundation for QTL mapping, QTL map-based cloning,superior gene screening and molecular marker-assisted breeding of essential agronomic traits.256 pairs of SRAP primers and 64 pairs of SSR primers of cotton were screened twice, 150 F2 derived from two kenaf varities,Thailand No.1 and F71,as mapping material.73 pairs of SRAP primers and 8 pairs of SSR primers were screened,and these primers are clearly visible and high poly-morphic. A genetic linkage map with 128 DNA loci spanning 2155.43 cM was constructed. It contains 128 polymorphic makers scattered in 18 linkage groups with average interval of genetic linkage map 16.09 cM.In this paper,it is proved that SSR primers of cotton used for kenaf are available and genetic linkage map for kenaf has relatively high density and its markers are evenly distributed,which will be suitable for QTL mapping and QTL map-based cloning and etc. in the subsequent study.关键词
遗传连锁图谱/红麻/SRAP/SSRKey words
genetic linkage map/kenaf/SRAP/SSR分类
农业科技引用本文复制引用
武耀龙,李德芳,李建军,黄思齐,李辉,唐慧娟,陈安国..利用SRAP和SSR标记构建红麻遗传连锁图谱[J].中国麻业科学,2016,38(6):249-257,267,10.基金项目
国家麻类产业技术体系 （）
