中国全科医学2016，Vol.19Issue(18)：2126-2129,4.DOI:10.3969/j.issn.1007-9572.2016.18.003
辅助性 T 淋巴细胞17/调节性 T 淋巴细胞平衡在乙型肝炎病毒感染相关肝纤维化进展过程中的作用研究
Study on the Role of the Balance Th17/Treg in the Development of Hepatitis B Virus - related Liver Fibrosis
摘要
Abstract
Objective To explore the role of the balance between T helper 17 cells( Th17) / regulatory T cells (Treg)in the development of hepatitis B virus - related(HBV - related)liver fibrosis. Methods A total of 104 HBV - infected people from the First Affiliated Hospital of Kunming Medical University were recruited from January 2011 to June 2015,all patients had no antiviral treatment history. Based on the liver stiffness measurements(LSM),all patients were divided into mild hepatic fibrosis group(LSM < 9. 7 kPa,29 cases),moderate liver fibrosis group(9. 7 kPa≤LSM < 17. 5 kPa,53 cases)and severe liver fibrosis group( LSM ≥17. 5 kPa,22 cases). The sex,age,family history of hepatitis B,body mass index (BMI),and alanine aminotransferase(ALT)were recorded in all patients. To find cell frequency of Th17 and Treg in peripheral blood by using flow cytometry,then the ratio of Th17 / Treg was calculated. Results There was no significant difference in the sex,family history of hepatitis B and BMI among the three groups(P > 0. 05). The age of moderate hepatic fibrosis group and severe liver fibrosis group were higher than that in mild hepatic fibrosis group,and ALT levels were higher than that in the mild hepatic fibrosis group(P < 0. 05). The age of severe liver fibrosis group was higher than that in moderate hepatic fibrosis group (P < 0. 05). Th17 frequency and the ratio of Th17 to Treg in moderate liver fibrosis group was significantly higher than those in mild liver fibrosis group(P < 0. 05);Treg frequency in severe liver fibrosis group was significantly lower than that in mild liver fibrosis group,but the ratio of Th17 to Treg in severe liver fibrosis group was significantly higher than that in mild liver fibrosis group(P < 0. 05). Th17 frequency was 2. 5% (3. 6% ),Treg frequency was 1. 0% (1. 0% ),ALT level was 47. 2(36. 2) U/ L,LSM was 12. 8(6. 9)kPa. The frequency of Th17 were positively correlated with frequency of Treg( rs = 0. 231,P= 0. 018);there was no linear correlation between the LSM and the Th17 frequency(rs = 0. 162,P = 0. 428)or ALT levels(rs= 0. 201,P = 0. 526);the frequency of Treg was negatively correlated with LSM( rs = - 0. 215,P = 0. 041);the ratio of Th17 / Treg was positively correlated with LSM(rs = 0. 268,P = 0. 019). Conclusion In the development of HBV - related liver fibrosis,the regulatory effects of Th17 was not clear,Treg have obvious regulatory effects,the balance Th17 / Treg has important significance.关键词
乙型肝炎病毒/肝纤维化/T 淋巴细胞,辅助/T 淋巴细胞,调节性Key words
Hepatitis B virus/Liver fibrosis/T - lymphocytes,helper/T - lymphocytes,regulatory分类
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刘怀鄂,游晶,洪敏,张艳梅,张禄,陆丽蓉,张茹薏,丁洁..辅助性 T 淋巴细胞17/调节性 T 淋巴细胞平衡在乙型肝炎病毒感染相关肝纤维化进展过程中的作用研究[J].中国全科医学,2016,19(18):2126-2129,4.基金项目
云南省科技厅应用基础研究面上项目 （）
