中国全科医学2016，Vol.19Issue(18)：2130-2134,5.DOI:10.3969/j.issn.1007-9572.2016.18.004
乙型肝炎肝硬化患者辅助性 T 淋巴细胞17和调节性 T 淋巴细胞及维甲酸相关核孤儿受体γt 和叉状头/翅膀状螺旋转录因子3 mRNA 表达水平的变化及其临床意义研究
Changes of Th17 and Treg and the Expressions of RORγt and Foxp3 mRNA in Patients With HBV Related Liver Cirrhosis and the Clinical Significance
摘要
Abstract
Objective To analyze the changes of peripheral Th17 and Treg and the expressions of RORγt and Foxp3 mRNA in patients with HBV related liver cirrhosis and explore the clinical significance. Methods We enrolled 64 patients with chronic HBV in fection who received treatment in the First Affiliated Hospital of Kunming Medical University from July to December in 2015. Among them,21 patients with chronic hepatitis B were assigned into CHB group,21 patients at compensated liver cirrhosis were assigned into CLC group,and 22 patients at decompensated liver cirrhosis were assigned into DCLC group. 20 healthy people who received physical examination in the hospital in the same period were enrolled as control group. The general data(gender,age,ALT,AST and TBIL)were collected;expression rates of Th17 and Treg in the peripheral blood of the subjects were detected using flow cytometry,and the ratio of Th17 / Treg was calculated. RT-qPCR method was applied to determine the expressions of RORγt and Foxp3 mRNA of peripheral blood. Results The four groups were significantly different in age,ALT,AST and TBIL( P < 0. 05). CHB group,CLC group and DCLC group were higher than control group in the expression rates of Th17 and Treg and Th17 / Treg(P < 0. 05);DCLC group was higher than CHB group in the expression rates of Th17 and Treg and Th17 / Treg,and was higher than CLC group in the expression rate of Th17 and Th17 / Treg(P < 0. 05). CHB group,CLC group and DCLC group were higher than control group in the expressions of RORγt mRNA and Foxp3 mRNA (P < 0. 05);DCLC group was higher than CHB and CLC group in the expressions of RORγt mRNA and Foxp3 mRNA(P <0. 05). For CHB group,CLC group and DCLC group,the expression rate of Th17 was positively correlated with the expression of RORγt mRNA( P < 0. 05);for CHB group,CLC group and DCLC group,the expression rate of Treg was positively correlated with the expression of Foxp3 mRNA(P < 0. 05);for CHB group,CLC group and DCLC group,the expression rate of Th17 and the expression of RORγt mRNA was positively correlated with ALT(P < 0. 05);for CHB group,the expression of Foxp3 mRNA was positively correlated with ALT( P < 0. 05);for CHB group,the expressions of RORγt mRNA and Foxp3 mRNA were positively correlated with AST and TBIL( P < 0. 05 ) . Conclusion Th17 and Treg cells and their specific transcription factors RORγt and Foxp3 may be related with the progress of HBV - related liver cirrhosis and liver function.关键词
乙型肝炎/肝硬化/T 淋巴细胞,辅助/T 淋巴细胞,调节性/维甲酸相关核孤儿受体 γt/叉状头/ 翅膀状螺旋转录因子 3Key words
Hepatitis B/Liver cirrhosis/T - lymphocytes,helper/T - lymphocytes,regulatory/RORγt/Foxp3分类
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涂琛,游晶,刘怀鄂,陈红英,洪敏,刘子杰,娄鸣..乙型肝炎肝硬化患者辅助性 T 淋巴细胞17和调节性 T 淋巴细胞及维甲酸相关核孤儿受体γt 和叉状头/翅膀状螺旋转录因子3 mRNA 表达水平的变化及其临床意义研究[J].中国全科医学,2016,19(18):2130-2134,5.基金项目
云南省科技计划项目（2011WS0053；2011FZ121；2012WS0028）；云南省教育厅基金项目 （）
