中国全科医学2016，Vol.19Issue(18)：2201-2205,5.DOI:10.3969/j.issn.1007-9572.2016.18.018
脾气虚和脾阳虚模型大鼠脑肠肽与下丘脑葡萄糖转运体1及葡萄糖转运体3表达水平变化的实验研究
Change of Expression Levels of Glucose Transporter - 1 and Glucose Transporter - 3 in Hypothalamus and Brain - gut Peptide of Spleen-qi Deficiency Rats and Spleen-yang Deficiency Rats
摘要
Abstract
Objective Analysis the changes of the expression levels of brain - gut peptide〔β - endorplhin(β-EP), cholecystokinin(CCK)and vasoactive peptide( VIP)〕and glucose transporter - 1( GLUT1)and glucose transporter - 3 (GLUT3)in hypothalamus of spleen deficiency rats. Methods From March to September 2015,a total of 24 SPF male SD rats were divided into control group,spleen - qi deficiency group and spleen - yang deficiency group with 8 rats in each group by random number table method. The model rats of spleen - qi deficiency were built by immoderate diet and overfatigue;the model rats of spleen - yang deficiency were built by bitter and cold aloes on the basis of the model rats of spleen - qi deficiency. The three groups were observed by general information( signs and state,body mass,temperature and food - intake)and grip of forelegs. The expression levels of β-EP,CCK and VIP in hypothalamus,stomach and jejunum were assessed by enzyme linked immunosorbent assay(ELISA);the mRNA levels of GLUT1 and GLUT3 were measured by real time quantitative fluorescent polymerase chain reaction( Real - time PCR);the levels of GLUT1 and GLUT3 in hypothalamus were measured by Western blotting. Results The body mass,food - intake and grip of forelegs of spleen - qi deficiency group were lower than those of control group(P < 0. 05);the body mass,temperature,food - intake and grip of forelegs of spleen - yang deficiency group were lower than those of control group(P < 0. 05);the temperature of spleen - yang deficiency group was lower than that of spleen -qi deficiency group(P < 0. 05). The expression level of β-EP in hypothalamus of spleen - qi deficiency group was lower than that of control group(P < 0. 05);the expression level of β-EP in stomach and jejunum and the expression levels of CCK and VIP in hypothalamus,stomach and jejunum of spleen - qi deficiency group were higher than those of control group(P < 0. 05). The expression level of β-EP in hypothalamus of spleen - yang deficiency group was lower than that of control group and the expression levels of β-EP and CCK in stomach and jejunum and the expression level of VIP in hypothalamus,stomach and jejunum of spleen- yang deficiency group were higher than those of control group(P < 0. 05);the expression level of β-EP in hypothalamus and the expression level of CCK in hypothalamus and jejunum of spleen - yang deficiency group were lower than those of spleen - qi deficiency group and the expression level of β-EP in stomach and jejunum and the expression level of VIP in hypothalamus, stomach and jejunum of spleen - yang deficiency group were higher than those of spleen - qi deficiency group(P < 0. 05). The mRNA expression and protein expression of GLUT1 and GLUT3 in hypothalamus of spleen - qi deficiency group and spleen - yang deficiency group were lower than those of control group(P < 0. 05);the mRNA expression and protein expression of GLUT1 and GLUT3 in hypothalamus of spleen - yang deficiency group were lower than those of spleen - qi deficiency group(P < 0. 05). Conclusion Under the condition of spleen deficiency,the mRNA expression levels of GLUT1 and GLUT3 in hypothalamus are lower and the expression levels of β-EP,CCK and VIP in hypothalamus,stomach and jejunum are abnormal,which may be a new viewpoint of studying the essence of spleen deficiency.关键词
脾气虚/脾阳虚/脑肠肽/葡萄糖转运蛋白质/下丘脑Key words
Spleen - qi deficiency/Spleen - yang deficiency/Brain - gut peptide/Glucose transport protein/Hypothalamus分类
医药卫生引用本文复制引用
丛培玮,尚冰,王艳杰,赵丹玉,张林,王德山..脾气虚和脾阳虚模型大鼠脑肠肽与下丘脑葡萄糖转运体1及葡萄糖转运体3表达水平变化的实验研究[J].中国全科医学,2016,19(18):2201-2205,5.基金项目
国家重点基础研究发展计划（973计划）课题 （）
