肖扬 1王锋锋 2熊杰 1孟磊 2姚睿智 2王峻 2庄礼兴 2陈铭2
作者信息
- 1. 510120 广东省广州市，广州中医药大学第一临床医学院
- 2. 广州中医药大学第一附属医院
- 折叠
摘要
Abstract
Objective To study the analgesic effect and mechanism of electroacupuncture(EA)acupoints in renal colic rats. Methods From March 31,2015 to April 28,2015,26 male SD rats in a SPF grade were selected and divided into normal group( n = 8),model group( n = 8)and EA group( n = 10)by random number table method. The rats were fed adaptively for 7 days. From the 8th day,the rats in normal group were free to drink water,the rats of another groups were free to drink stone inducing agent(1% ethylene glycol solution and 1% ammonium chloride aqueous solution)for 10 days to establish renal colic models induced by calcium oxalate stone. There were two deaths including one in model group and one in EA group after models were established successfully. The electroacupuncture was applied to “ Shenshu“ ( BL23 ),“ Sanjiaoshu“(BL22),“ Sanyinjiao“(SP6)and “ Shuiquan“(KI5)in the rats in EA group for 3 days. Rats were executed on the next day after treatment ended. Before treatment and on day 1,2,3 of treatment,pain threshold value was compared among the three groups,spontaneous pain score was compared between model group and EA group,the TXA2,PGI2 and 5 - HT expressions among the three groups were detected by ELISA method,the pathological changes of the left kidney tissue of each group were observed after biopsies. Results There was interaction effect between treatment measure and time in pain threshold value(P <0. 05);the main effect between treatment measure and time was significant in pain threshold value(P < 0. 05);Rats in model group and EA group had lower pain threshold than normal group before treatment,and on the first and second day of treatment(P< 0. 05);the rats in model group had lower pain threshold than normal group on the third day of treatment(P < 0. 05);the rats in EA group had higher pain threshold than model group on day 1,2,3 of treatment(P < 0. 05). There was interaction effect between treatment measure and time in the score of spontaneous pain(P < 0. 05);the main effect between treatment measure and time was significant in the score of spontaneous pain(P < 0. 05);the rats in EA group had lower score of spontaneous pain than model group on day 1,2,3 of treatment( P < 0. 05). The rats of model group were higher than normal group in the expressions of TXA2,PGI2 and 5-HT in renal tissue,and EA group was higher than normal group in the expression of PGI2 in renal tissue(P < 0. 05);the rats of EA group were lower than model group in the expressions of TXA2,PGI2 and 5-HT in renal tissue(P < 0. 05). For normal group,no calcium oxalate crystal deposit was observed in kidney;glomerular morphology was regular;no congestion in surrounding tissue,no infiltration of inflammatory cells,and no expansion of kidney tubules were found;the cells were in alignment and clear structure. For model group,glomerular morphology was regular;congestion and edema in surrounding tissue,infiltration of inflammatory cells,obvious expansion of kidney tubules,and calcium oxalate crystal deposit were noted;the cells were in disorder,and epithelial cells fell off and were necrotic. For EA group,glomerular morphology was regular;slight congestion and infiltration of inflammatory cells in surrounding tissue,and expansion of kidney tubules were noted,but no calcium oxalate crystal deposit was observed;the cells were in disorder and clear structure;no epithelial cells fell off and were necrotic. Conclusion The EA acupoints treatment has analgesic effect on renal colic rats. The therapeutic mechanism is possibly achieved by inhibiting the expressions of inflammatory mediators TXA2, PGI2 and neurotransmitter 5-HT.关键词
电针/肾绞痛/大鼠/针刺镇痛/血栓素 A2/前列环素/血清素Key words
Electroacupuncture/Renal colic/Rats/Acupuncture Analgesia/Thromboxane A2/Epoprostenol/Serotonin分类
医药卫生
