陈苏 1杜潘艳 2郑红 2王宝林 2高翠红 2张双2
作者信息
- 1. 063000 河北省唐山市第四医院检验科
- 2. 唐山市妇幼保健院检验科
- 折叠
摘要
Abstract
Objective To analyze the viral load and clinical features of children with hand,foot and mouth disease (HFMD)by EV71 and CoxA16. Methods A total of 430 children who were definitely diagnosed with HFMD in the Department of Pediatrics in Tangshan Women and Children Health - care Hospital from May to September in 2015 were enrolled. According to clinical symptoms,physical signs and species of virus,the children were divided into EV71 mild group(162 cases),EV71 severe group(87 cases),CoxA16 mild group(158 cases)and CoxA16 severe group(23 cases). Throat swabs of the children were collected and the RNA of EV71 and CoxA16 was detected by real - time fluorescent quantitative RT-qPCR method,and viral load was calculated. Length of disease,temperature,length of fever,dental ulcer,salivation,cough,hand rash,foot rash, somnolence,convulsion,vomit,change of consciousness,limb jitter,and myospasm of the children were recorded. Results Cycle threshold(Ct value)shown by standard curve had highly negative correlation with the viral load( r = - 1. 000,P <0. 01). The relation between the Ct value(X)and the logarithm of viral load(Y)was Y = - 0. 29X + 13. 03 . CoxA16 mild group and CoxA16 severe group were lower than EV71 mild group and EV71 severe group in Ct value(P < 0. 05). There was no significant difference in the Ct value of HFMD children between EV71 mild group and EV71 severe group and between CoxA16 mild group and CoxA16 severe group(P > 0. 05). EV71 severe group and CoxA16 severe group had longer length of disease than EV71 mild group and CoxA16 mild group(P < 0. 05). EV71 severe group had longer length of disease than CoxA16 severe group(P < 0. 05). The four groups were not significantly different in the incidence rates of temperature ≥38. 5 ℃ ,dental ulcer,salivation,hand rash and foot rash(P > 0. 05). EV71 severe group and CoxA16 severe group had higher incidence rate of the length of fever ≥3 d than EV71 mild group and CoxA16 mild group(P < 0. 007). EV71 severe group and CoxA16 severe group had higher incidence rate of temperature ≥38. 5 ℃ plus length of fever ≥3 d than EV71 mild group and CoxA16 mild group,EV71 mild group was higher than CoxA16 mild group in the incidence rate of temperature ≥38. 5 ℃ plus length of fever≥3 d,and EV71 severe group was higher than CoxA16 severe group in the incidence rate of temperature ≥38. 5 ℃ plus length of fever ≥3 d(P < 0. 007). CoxA16 mild group and CoxA16 severe group had higher incidence rates of cough,the number of hand rashes ≥15(both hands)and the number of foot rashes ≥15(both feet)than EV71 mild group and EV71 severe group (P < 0. 007). EV71 severe group and CoxA16 severe group had higher incidence rates of somnolence and vomit than EV71 mild group,and EV71 severe group was higher than CoxA16 severe group in the incidence rates of somnolence( P < 0. 01 ). Conclusion The viral load of HFMD children varies with different types of enterovirus but is not different among different disease severity levels. HFMD children with different types of enterovirus and disease severity levels are different in the incidence rates of length of fever ≥3 d,temperature ≥38. 5 ℃ plus length of fever ≥3 d,cough,the number of hand rashes ≥15(both hands) and the number of foot rashes ≥15( both feet),somnolence,convulsion,vomit,change of consciousness,limb jitter and myospasm.关键词
手足口病/肠道病毒属/柯萨奇病毒感染/病毒载量/体征和症状Key words
Hand, foot and mouth disease/Enterovirus/Coxsackievirus infections/Viral load/Signs and symptoms分类
医药卫生
