摘要
Abstract
Objective:To explore the results of ELISA method and fluorescence quantitative PCR HCV-RNA for detection of anti-HCV and HCV-RNA in serum in hepatitis C.Methods:Serum of 102 patients with chronic hepatitis C was collected.They were detected by ELISA method and fluorescence quantitative PCR HCV-RNA.Results:In 102 patients,74 cases were in anti-HCV(+) group,28 cases were in anti-HCV(-) group,and the detection rate was 27.5%(28/102).In HCV-RNA detection,in 74 cases,the virus content of 54 cases were more than 104 IU/mL,and the detection rate was 73%(54/74);in 28 cases,the virus content of 3 cases were more than 104 IU/mL,and the detection rate was 10.7%(3/28);there was significant difference between groups(P<0.05).Conclusion:The combined detection of anti-HCV and HCV-RNA can improve the detection rate of hepatitis C patients.By comparing the results of the test results,anti-HCV had misdiagnosis phenomenon in the diagnosis of hepatitis C patients,in fluorescence quantitative PCR HCV-RNA,the specificity was high,and sensitivity was strong.It was an important basis for clinical diagnosis of hepatitis C.关键词
荧光定量PCR/丙型肝炎/抗-HCV/HCV-RNAKey words
Fluorescence quantitative PCR/Hepatitis c/Anti-HCV/HCV-RNA
