中国药房2016，Vol.27Issue(18)：2532-2534,3.DOI:10.6039/j.issn.1001-0408.2016.18.28
RP-HPLC法同时测定荷叶颗粒中3种有效成分的含量
Simultaneous Determination of Three Active Compounds in Heye Granule by RP-HPLC
摘要
Abstract
OBJECTIVE:To establish a method for simultaneous determination of rutin,quercetin and nuciferine in Heye gran-ule. METHODS:HPLC was performed on the column of Hypersil ODS2 with mobile phase of acetonitrile-water(containing 0.3%phosphoric acid and 0.4% triethylamine)(gradient elution)at a flow rate of 1.0 ml/min,the detection wavelength was 256 nm,the column temperature was 25 ℃,and the injection volume was 10 μl. RESULTS:The linear range was 0.012-0.240 μg for rutin(r=0.999 9),0.010 4-0.208 μg for quercetin(r=0.999 9)and 0.015-0.300 μg for nuciferine(r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 2.0%;recoveries were 98.5%-101.3%(RSD=1.1%,n=6),99.1%-101.6%(RSD=1.0%, n=6)and 98.9%-101.7%(RSD=1.2%,n=6). CONCLUSIONS:The method is simple,accurate and reliable,and can provide ref-erence for quality control of Heye granule.关键词
荷叶颗粒/芦丁/槲皮素/荷叶碱/反相高效液相色谱法Key words
Heye granule/Rutin/Quercetin/Nuciferine/RP-HPLC分类
医药卫生引用本文复制引用
李梦杰,刘苏珍,周斌,陈振华..RP-HPLC法同时测定荷叶颗粒中3种有效成分的含量[J].中国药房,2016,27(18):2532-2534,3.基金项目
国家自然科学基金资助项目 （）
