草业学报2016，Vol.25Issue(6)：94-101,8.DOI:10.11686/cyxb2015410
利用 SSR 分子标记构建四倍体杂交冰草的遗传连锁图谱
Construction of a genetic linkage map for tetraploid hybrid wheatgrass using a SSR molecular marker
摘要
Abstract
To establish a genetic linkage map in tetraploid hybrid wheatgrass genetic mapping was conducted u-sing a simple sequence repeats (SSR)molecular marker technique with ‘Joinmap’4.0 software.347 individu-als from the F2 segregating population and their parents were utilized,this helped lay the foundation for further study of marker-assisted breeding,and quantitative trait locus (QTL)location of important traits in wheat-grass,such as disease resistance and yield.Thirty optimal primers with clear,stable and high polymorphic bands were screened from 256 tested SSR primers.A total of 224 SSR loci were obtained from polymerase chain reaction (PCR)amplification with an average of 7.47 loci per primer,of which 185 were polymorphic lo-ci,accounting for 82.6% of all loci.Segregation distortion analysis showed that a total of 24 loci were distort-ed,accounted for 13.0% of all (185)polymorphic loci,which met the requirement of <30% segregation dis-tortion for plant genetic mapping.These polymorphic loci would be useful for genetic mapping.In this study, a molecular genetic linkage map of tetraploid hybrid wheatgrass was constructed which contained 185 loci and 14 linkage groups with length ranging from 123.0 to 202.6 cM.The longest and the shortest linkage group were LG4 and LG12,respectively.The average length of each linkage group was 167.32 cM,total length of the genome was 2342.5 cM and the average marker distance was 12.66 cM.关键词
杂交冰草/四倍体/SSR 标记/遗传连锁图谱Key words
hybrid wheatgrass/tetraploid/SSR marker/genetic linkage map引用本文复制引用
姜志艳,于肖夏,于卓,张志成,石悦,姜超..利用 SSR 分子标记构建四倍体杂交冰草的遗传连锁图谱[J].草业学报,2016,25(6):94-101,8.基金项目
国家自然科学基金面上项目（31172254）资助。 （）
