草业学报2016，Vol.25Issue(6)：102-108,7.DOI:10.11686/cyxb2015532
农杆菌侵染条件对柱花草遗传转化效率的影响
Factors affecting genetic transformation efficiency for stylo (Stylosanthes guianensis) with Agrobacterium tumefaciens
摘要
Abstract
The objective of this study was to investigate effects of Agrobacterium concentration,infection time and co-culture duration on the genetic transformation efficiency for stylo (Stylosanthes guianensis )using a high-efficiency regeneration system that had been developed previously.Hypocotyls from stylo seedlings were used as plant material and theβ-glucuronidase (GUS)gene was used as the reporter gene.The callus transfor-mation efficiency was evaluated by GUS staining.The callus transformation efficiency reached 72% under the following conditions:Agrobacterium at a concentration of OD600 =0.4-0.6,with 15 mins infection and 3 days of co-cultivation.The exogenous gene had been successfully introduced into the stylo genome as indicated by GUS staining and PCR detection after callus selection,shoot differentiation and rooting.The results indicate that this A.tu-mefaciens-mediated transformation system is suitable for genetic improvement and functional gene analysis of stylo.关键词
根癌农杆菌/遗传转化/GUS 染色/柱花草Key words
Agrobacterium tumefaciens/genetic transformation/GUS staining/Stylosanthes引用本文复制引用
陈彩虹,钏秀娟,王荟,贾艳星,陈志坚,刘国道,罗丽娟..农杆菌侵染条件对柱花草遗传转化效率的影响[J].草业学报,2016,25(6):102-108,7.基金项目
国家自然科学基金（31360575），国家牧草产业技术体系热带牧草育种（CARS-35-03），海南大学优秀研究生学位论文培育计划（M8K3124001001003002）资助。 （）
