草业学报2016，Vol.25Issue(6)：118-125,8.DOI:10.11686/cyxb2015419
鸭茅香柱菌形态学、生理学及系统发育研究
Morphological,physiological and phylogenetic characteristics of Epichloёendophyte associated with Dactylis glomerata
摘要
Abstract
Surveys conducted in Xinjiang revealed a Dactylis glomerata population infected by Epichloё endo-phyte.The conidial stroma on the grass tillers were waxy,yellow and 45 -55 mm in length.The endophyte was isolated and incubated on PDA (potato dextrose agar)medium for two weeks at 25℃.The diameter of col-onies ranged from 45-54 mm.They were compact,centrally raised with a white cottony mass of aerial hyphae and white to yellow on the underside.Hyphae were long with septum and conidia very sparse unless the colony was stressed.Conidiophores were hyaline,smooth,13 -33 μm long,2.7-4.1 μm at base and less than 1.0μm at the tip.Conidia were hyaline,oval to reniform and size ranged;(4.1 ±0.5 )μm ×(2.2 ±0.5 )μm. These dimensions are typical of Epichloёendophytes.The preference of these isolated Epichloёendophytes for carbon source was sucrose>fructose>glucose>starch and for nitrogen sources,ammonium nitrate>peptone>potassium nitrate.These isolations exhibited differences in the hydrolysis of casein and starch.ITS sequence of isolation-Ed0907 was analysis and the phylogenetic tree constructed;it showed that the tested isolation clus-tered with Epichloёtyphina with 100% similarity rate.Based on these morphological,physiological and phylo-genetic characteristics,the endophyte isolated from D .glomerata was identified as Epichloёspp.关键词
内生真菌/Epichloё-Dactylis glomerata 共生体/培养特征/ITS 序列Key words
endophyte/Epichloё-Dactylis glomerata symbionts/culture character/ITS sequence引用本文复制引用
王正凤,李秀璋,李春杰..鸭茅香柱菌形态学、生理学及系统发育研究[J].草业学报,2016,25(6):118-125,8.基金项目
国家973计划课题（2014CB138702）和国家自然科学基金项目（31372366）资助。 （）
