发光学报2016，Vol.37Issue(6)：744-750,7.DOI:10.3788/fgxb20163706.0744
邻菲啰啉和水杨酸构筑铜配合物的电化学行为及与DNA的相互作用
Electrochemical Property and Interaction of DNA with 1,10-phenanthroline and Salicylic Acid Copper Complex
摘要
Abstract
A new ternary complex of [Cu(phen)(SA)2]·H2O (phen =1,10-phenanthroline, SA=salicylic acid) was synthesized and the electrochemical behavior was studied. In addition, with hsDNA as the research target, the interaction between the complex and hsDNA was investigated by UV absorption spectroscopy, CV method, differential pulse voltammetry ( DPV) , and DNA viscosity titration experiment. The experiment results indicate that—COOH of SA and N of phen are liganded with Cu2+. Cyclic voltammetric shows that the complex has one pairs of redox peaks at 0. 489 V/0. 050 V, and the reaction of Cu2 +on the electrode is controlled by diffusion. With the addition of hsDNA, the UV absorption peaks of complex red-shift and the intensity is weakened, the peak cur-rent decreases significantly and the potential moves positively, and the relative viscosity of hsDNA increases. It’s concluded that [Cu(phen)(SA)2]·H2O and hsDNA bind by the mode of intercala-tion, the combining ratio and the binding constant are 1∶1 and 1. 55 × 104 L·mol-1 , respectively.关键词
水杨酸/1,10-邻菲啰啉/铜/DNA/相互作用Key words
salicylic acid/1,10-phenanthroline/copper (II)/DNA/interaction分类
化学化工引用本文复制引用
李小芳,冯小强,杨声,朱元成..邻菲啰啉和水杨酸构筑铜配合物的电化学行为及与DNA的相互作用[J].发光学报,2016,37(6):744-750,7.基金项目
国家自然科学基金(51063006) （51063006）
天水师范学院“青蓝人才”工程和中青年教师科研项目(TSA1508)资助 （TSA1508）
