华南农业大学学报2016，Vol.37Issue(4)：32-37,6.DOI:10.7671/j.issn.1001-411X.2016.04.006
球孢白僵菌对亚洲玉米螟血细胞毒力和形态的影响
Effects of Beauveria bassiana on virulence and cellular morphology of hemocytes of Ostrinia furnacalis
摘要
Abstract
[Objective] To test the effect of Beauveria bassiana on SYSU-OfHe-C, a cell line from hemo-cytes of Ostrinia furnacalis. To provide a theory basis for application of B. bassiana in the prevention and treatment of O. furnacalis.[Method] MTT and CCK-8 were employed to test the virulence of B. bassiana on SYSU-OfHe-C. Optical microscope and scanning electron microscope were used to observe the growth of B. bassiana and its influence on the morphology of SYSU-OfHe-C cells. [Result]The IC50 values of B. bassiana against SYSU-OfHe-C cells at 24 hours after treatment were recorded as 2. 8 × 105 and 1. 4 × 105 mL-1 respectively by MTT and CCK-8. Furthermore, under real-time monitoring with optical micro-scope, encapsulization and phagocytosis were not found and B. bassiana was highly competitive when the conidia of B. bassiana were inoculated in the culture of SYSU-OfHe-C cells at logarithmic stage reaching a final concentration of 3. 5 × 104 mL-1 for B. bassiana. B. bassiana started to germinate at 10 hours after treatment, and meanwhile, SYSU-OfHe-C cells started to reduce in number and change in morphology. At 24 hours after treatment, B. bassiana grew the entire visual field. The nearly round SYSU-OfHe-C cells had elongated or turned into irregular shape. Aggregation, fragmentation, cavitation, protuberance and penetration of SYSU-OfHe-C cells caused by B. bassiana mycelia were observed.[Conclusion]B. bassiana can change cellular morphology of hemocytes of O. furnacalis, but cannot induce its immune responses.关键词
球孢白僵菌/亚洲玉米螟/血细胞/SYSU-OfHe-C/MTT法/CCK-8法Key words
Beauveria bassiana/Ostrinia furnacalis/hemocyte/SYSU-OfHe-C/MTT method/CCK-8 method分类
农业科技引用本文复制引用
李福霞,胡维娜,胡琼波..球孢白僵菌对亚洲玉米螟血细胞毒力和形态的影响[J].华南农业大学学报,2016,37(4):32-37,6.基金项目
公益性行业(林业)科研专项(201304408) （林业）
