继续医学教育2016，Vol.30Issue(6)：139-140,2.DOI:10.3969/j.issn.1004-6763.2016.06.083
叶酸缺乏致大鼠巨幼细胞性贫血的相关检测指标
The Related Detection Indicators of Megaloblastic Anemia in Rats Caused by Folic Acid Deifciency
摘要
Abstract
Objective To investigate the related detection indicators of megaloblastic anemia in rats caused by folic acid deficiency, including mean corpuscular volume(MCV), hemogram, myelogram.Methods 20 adult male SD rats were divided into 2 groups randomly, folic acid deifcient group(A group),folic acid normal group (B group). Detect serum folic acid and MCV at the beginning, then after 8-week feed, detect these two indicators again, and make the peripheral blood smear and bone marrow smear to observe the changes of hemogram and myelogram.Results For A group, 8 weeks later, the concentration of serum folic acid(6.05±1.67) µɡ/L) was obviously lower than the beginning index(12.76±2.03) µɡ/L), the difference had statistically signiifcance, MCV increased signiifcantly, the phenomenon of hypersegmentation of neutrophil nuclei appeared, the percent of megaloblasts in bone marrow was over 10%, as well as megaloblastic change in granular leukocytes and segmented megacarycyte occurred.Conclusion For megaloblastic anemia caused by folic acid deifciency, the peripheral blood smear appeared to be more large oval red blood cells and hypersegmentation of neutrophil nuclei. The bone marrow smear showed to be active proliferation of bone marrow cells, obvious proliferation in erythroid cells, megaloblastic change in every series of cells, especially in erythroid cells.关键词
叶酸/巨幼细胞性贫血/大鼠Key words
Folic acid/Megaloblastic anemia/Rats分类
医药卫生引用本文复制引用
闫文强,周迎,姚宏,韩红柳,柴天赐,王飞通..叶酸缺乏致大鼠巨幼细胞性贫血的相关检测指标[J].继续医学教育,2016,30(6):139-140,2.基金项目
江苏省大学生创新创业训练计划基金资助项目 （）
