南京林业大学学报（自然科学版）2016，Vol.40Issue(4)：56-62,7.DOI:10.3969/j.issn.1000-2006.2016.04.009
不同分布地天女木兰叶表皮微形态研究
Micromorphological characters of leaf epidermis in Magnolia sieboldii from different habitats
摘要
Abstract
Magnolia sieboldii is an endangered, rare woody plant species, with a discontinuous natural distribution and high values in perfume, pharmaceutical, food and ornamental industries. To explore genetic variations within⁃species and relationships among different habitats, we used scanning electronic microscope, principal component analysis, and cluster analysis to quantify the micromorphological characters of leaf epidermis among M. sieboldii populations from seven habitats. The results showed that there were two types of epidermal cells (shallow wavy and deep wavy), two types of stomatal apparatuses ( elliptically convex and concave) , two types of leaf epidermis crystals ( spherical and cylindrical) , three types of dense epidermal hairs ( unicellular, single⁃row bicellular and single⁃row multicellular) , as well as varia⁃tions in stomata size, density and epidermal hair length and density. The principal component analysis identified three principal components accounted for 87. 394% of the total variation in micromorphological characters of leaf epidermis. The cluster analysis showed that M. sieboldii from seven habitats belonged to two groups. The first group included the M. sieboldii from Shihu in Jilin Province and Zushan in Hebei Province, and this group had shallow wavy leaf epidermal cells, lower stomatal density, convex stomatal apparatus, short stomatal axis (<10 μm) and round leaf crystals. The second group included the M.sieboldii from Qingliangfeng in Zhejiang Province, Huangshan in Anhui Province, Leigong Mountain and Majiang in Guizhou Province and Maoershan in Guangxi Province. This group generally had deep wavy leaf epidermal cells, higher stomatal density, concave stomata, long stomatal axis (>10μm) and long cylindrical leaf crys⁃tals. These results suggested that the genetic relationships among different habitat in M. sieboldii were related to the geo⁃graphical distribution of the species and that the micromorphological characters of leaf epidermis could be used to study genetic variations and relationships among different habitat in M. sieboldii.关键词
天女木兰/叶表皮/微观形态/气孔Key words
Magnolia sieboldii/leaf epidermis/micro morphology/stoma分类
农业科技引用本文复制引用
张淑媛,杜凤国,王瑞俭,王金玲,孙广仁,高红兵..不同分布地天女木兰叶表皮微形态研究[J].南京林业大学学报（自然科学版）,2016,40(4):56-62,7.基金项目
国家林业公益性行业科研专项项目 （）
