南京林业大学学报（自然科学版）2016，Vol.40Issue(4)：63-68,6.DOI:10.3969/j.issn.1000-2006.2016.04.010
牛樟的组织培养和植株再生
Tissue culture and plant regeneration of Cinnamomum kanehirae Hay
摘要
Abstract
In order to establish a high efficient regeneration system of Cinnamomum kanehirae, its stems were used as explants to study the effects of collection season, sterilization methods and different hormone concentrations on stem re⁃generation in the processes of buds induction. The results were as follows:The lower infecting and browning rate, quic⁃ker differentiation speed and higher germination rate were observed when explants were collected in spring and summer. The infectious rate significantly decreased if the explants were treated by thiophanate⁃methyl solution diluted 1 000 times for 10 min, 75% alcohol for 30 s and 0.1% mercuric chloride for 8-10 min. And these treatments were harmless to ex⁃plants. MS was an optimal basic medium. The optimum medium for buds induction was MS+1.0 mg/L 6-BA+ 0.1 mg/L IBA, which exhibited higher bud induction rate and thicker buds. MS+ 1.5 mg/L 6-BA+ 0.1 mg/L IBA was the optimal medium for bud subculture proliferation, which exhibited higher cluster bud proliferation rate and strong bud. 1/2MS +0.2 mg/L ABT+ 0.05 mg/L IBA+0.5 g/L AC was the optimal medium for root differentiation, with the rooting percent⁃age 100% after 30 days. Establishment of stable plant regeneration system of C. kanehirae will be useful for saving the resources of endangered species and expanding its living space. Also, it will lay a foundation for the improvement of breeding and the genetic transformation system in the future.关键词
牛樟/组织培养/植株再生Key words
Cinnamomum kanehirae Hay/tissue culture/plant regeneration分类
农业科技引用本文复制引用
官锦燕,谭嘉娜,罗剑飘,黄海英,罗青文,杨俊贤,陈月桂..牛樟的组织培养和植株再生[J].南京林业大学学报（自然科学版）,2016,40(4):63-68,6.基金项目
广东省科技计划项目（2013B020415015，2014A030304012）；湛江市科技计划项目（2014A03020）；广东省工业技术研究院生物工程研究所科技基金项目（ C201307）；广东省糖－能甘蔗育种与产业技术创新重点科研基地二期项目 （）
