南京林业大学学报（自然科学版）2016，Vol.40Issue(4)：171-176,6.DOI:10.3969/j.issn.1000-2006.2016.04.027
松材线虫毒力及发育转型与细胞自噬关系研究
Research on the relationship of autophagy with the virulence and development of Bursaphelenchus xylophilus
摘要
Abstract
In order to identify the autophagy in Bursaphelenchus xylophilus, research on the relationship between autoph⁃agy and the different virulence and development of B. xylophilus was carried out in this paper. Transmission electron mi⁃croscopy (TEM) method was used to identify autophagy in B. xylophilus, and quantitative reverse transcription PCR ( qRT⁃PCR) was adopted to quantify the BxAtg1 and BxAtg8 at different virulence and at different stages of the develop⁃ment of B. xylophilus. The results from TEM observations showed that the process of autophagy existed in B. xylophilus after starvation⁃induced. The mRNA expression of BxAtg1 was decreased in different virulence nematodes which exposed to long⁃term starvation for 12, 24 and 36 h. The mRNA expression of BxAtg8 in strongly virulent nematodes was in⁃creased in 24 h, but the mRNA expression of BxAtg8 in weakly virulent nematodes was obviously increased in 36 h. And there was increment of both the mRNA expression of BxAtg1 and BxAtg8 in JⅢand JⅣcompared to the propagtive form of B. xylophilus. Compareing to the morphological characteristics of the different development of Bursaphelenchus xylophilus. These suggest that the mechanism of autophagy activity regulating may be related to the virulence of nematodes and auto⁃phagy assisted the nematodes in entering dispersal phase, which improved their ability to adapt to the environment at the same time. This study provided fundamental information the autophagy of B. xylophilus.关键词
松材线虫/细胞自噬/毒力/发育转型/透射电镜/实时定量PCRKey words
Bursaphelenchus xylophilus/autophagy/virulence/development/transmission electron microscopy/RT-qPCR分类
农业科技引用本文复制引用
邓丽娜,吴小芹..松材线虫毒力及发育转型与细胞自噬关系研究[J].南京林业大学学报（自然科学版）,2016,40(4):171-176,6.基金项目
国家自然科学基金项目（31270683）；江苏高校优势学科建设工程资助项目 （）
