山东农业科学2016，Vol.48Issue(6)：1-6,6.DOI:10.14083/j.issn.1001-4942.2016.06.001
大白菜光周期调控因子 CCA1的差异分析及功能标记开发
Differential Analysis and Functional Marker Development of Photoperiod Regulation Factor CCA1 from Two Chinese Cabbage Inbred Lines
摘要
Abstract
By the trend of photoperiod in spring and autumn,the sensibilities of two Chinese cabbage in-bred lines,06 -247 and He102,to photoperiod were identified.The 06 -247 was sensitive to long day, while He102 was opposite.The full -length cDNA sequence of photoperiod regulation factor CCA1 (Circadian clock associated 1 )from two lines were compared by homology -based cloning.It was revealed that there were two 6 -bp InDels and 14 non -synonymous SNPs between the CCA1 coding regions of 06 -247 and He102.The coding product had 552 and 556 amino acid residues respectively.The non -synonymous SNPs caused the exchange of amino acids with different property.The amino acid differences were mainly located in middle protein -protein interaction domain and C -terminal phosphorylation domain.The codominant molec-ular markers to distinguish the two 6 -bp InDels were developed and validated.The results provided founda-tion for further elucidating the molecular mechanism of photoperiodic response of CCA1 in Chinese cabbage.关键词
大白菜/依赖/不依赖长日照开花型/BraCCA1/插入/缺失突变/功能标记Key words
Chinese cabbage/Flowering relying (not)on long day/BraCCA1/Insertion /Deletion(In-Del)mutation/Functional marker分类
农业科技引用本文复制引用
张志刚,刘辰,刘栓桃,李巧云,王晓,王立华,赵智中,王淑芬,徐文玲,刘贤娴..大白菜光周期调控因子 CCA1的差异分析及功能标记开发[J].山东农业科学,2016,48(6):1-6,6.基金项目
山东省自然科学基金项目（ZR2014CM021、ZR2015YL073）；山东省农业科学院青年基金项目（2014QNM11）；山东省农业良种工程项目“十字花科名产蔬菜新品种选育” （）
